PMID- 17307191
OWN - NLM
STAT- MEDLINE
DCOM- 20070430
LR  - 20090115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1146
IP  - 1
DP  - 2007 Mar 30
TI  - High-performance liquid chromatography-atmospheric pressure chemical ionization 
      mass spectrometry and gas chromatography-flame ionization detection 
      characterization of Delta5-polyenoic fatty acids in triacylglycerols from conifer 
      seed oils.
PG  - 67-77
AB  - Edible conifer seeds can serve as a source of triacylglycerols (TGs) with unusual 
      Delta5 unsaturated polymethylene interrupted fatty acids (UPIFAs), such as 
      cis-5,9-octadecadienoic (taxoleic), cis-5,9,12-octadecatrienoic (pinolenic), 
      cis-5,11-eicosadienoic (keteleeronic) and cis-5,11,14-eicosatrienoic acids 
      (sciadonic). Conifer seed oils from European Larch (Larix decidua), Norway Spruce 
      (Picea abies) and European Silver Fir (Abies alba) have been analyzed by 
      non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) 
      with atmospheric pressure chemical ionisation (APCI)-MS detection. The influence 
      of different positions of double bonds in Delta5-UPIFAs on the retention and 
      fragmentation behavior is described and used for the successful identification of 
      TGs in each oil. TGs containing Delta5-UPIFAs have a higher retention in 
      comparison with common TGs found in plant oils with single methylene interrupted 
      Delta6(9)-FAs and also significantly changed relative abundances of fragment ions 
      in APCI mass spectra. Results obtained from HPLC/MS analyses are supported by 
      validated GC/FID analyses of fatty acid methyl esters after the 
      transesterification. The total content of Delta5-UPIFAs is about 32% for European 
      Larch, 27% for Norway Spruce and 20% for European Silver Fir. In total, 20 FAs 
      with acyl chain lengths from 16 to 24 carbon atoms and from 0 to 3 double bonds 
      have been identified in 64 triacylglycerols from 3 conifer seed oils.
FAU - Lisa, Miroslav
AU  - Lisa M
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Czech Republic.
FAU - Holcapek, Michal
AU  - Holcapek M
FAU - Rezanka, Tomas
AU  - Rezanka T
FAU - Kabatova, Nadezda
AU  - Kabatova N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070206
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Plant Oils)
RN  - 0 (Triglycerides)
RN  - 0 (delta5 polyunsaturated fatty acid)
SB  - IM
MH  - Atmospheric Pressure
MH  - Chromatography, Gas/instrumentation/*methods
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Fatty Acids, Unsaturated/*analysis
MH  - Flame Ionization
MH  - Larix/*chemistry
MH  - Plant Oils/*chemistry
MH  - Reproducibility of Results
MH  - Seeds/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/methods
MH  - Terminology as Topic
MH  - Triglycerides/*chemistry
EDAT- 2007/02/20 09:00
MHDA- 2007/05/01 09:00
CRDT- 2007/02/20 09:00
PHST- 2006/09/04 00:00 [received]
PHST- 2007/01/23 00:00 [revised]
PHST- 2007/01/25 00:00 [accepted]
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/05/01 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
AID - S0021-9673(07)00232-4 [pii]
AID - 10.1016/j.chroma.2007.01.122 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Mar 30;1146(1):67-77. doi: 10.1016/j.chroma.2007.01.122. 
      Epub 2007 Feb 6.