PMID- 17307800
OWN - NLM
STAT- MEDLINE
DCOM- 20070827
LR  - 20181211
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 19
IP  - 4
DP  - 2007 Apr
TI  - TLR2-dependent recognition of Streptococcus suis is modulated by the presence of 
      capsular polysaccharide which modifies macrophage responsiveness.
PG  - 375-89
AB  - Streptococcus suis capsular type 2 is an important swine pathogen and an agent of 
      zoonosis. Although meningitis is the most common form of disease, septicemia and 
      septic shock are also frequently reported. Despite reports that CD14 is involved 
      in the recognition of encapsulated S. suis by host cells, the mechanisms 
      underlying exacerbated release of pro-inflammatory cytokines, which may have a 
      negative impact on disease outcome, are unclear. Here, we demonstrated that 
      stimulation of human monocytes by whole encapsulated S. suis or its purified cell 
      wall components influences the relative expression of Toll-like receptor (TLR)-2 
      and CD14 mRNA. Moreover, this stimulation triggered the release of cytokines 
      (tumor necrosis factor-alpha, IL-1beta and IL-6) and chemokines (IL-8 and 
      monocyte chemoattractant protein-1), which was significantly reduced by 
      antibody-mediated blocking of TLR2 but not TLR4. Mouse macrophages deficient in 
      TLR2 also showed impaired cytokine responses to encapsulated bacteria. Given that 
      this response was completely abrogated in myeloid differentiation factor 88 
      (MyD88)-deficient macrophages, other TLRs might also be involved. Furthermore, we 
      demonstrated that the presence of capsular polysaccharide (CPS)-modulated S. suis 
      interactions with TLRs. In the absence of CPS, uncovered cell wall components 
      induced cytokine and chemokine production via TLR2-dependent as well as 
      -independent pathways, whereas CPS contributes to MCP-1 production in a 
      MyD88-independent manner. Overall, this study contributes to a better 
      understanding of the inflammatory processes induced by an encapsulated pathogen 
      and suggests that the relative expression of CPS, known to be modulated during 
      bacterial invasion and dissemination in the host, might alter interactions with 
      host cells and, consequently, the outcome of the inflammatory response.
FAU - Graveline, Richard
AU  - Graveline R
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche 
      en Infectiologie Porcine, Faculte de Medecine Veterinaire, Universite de 
      Montreal, 3200 rue Sicotte, St-Hyacinthe, Quebec, J2S 2M2, Canada.
FAU - Segura, Mariela
AU  - Segura M
FAU - Radzioch, Danuta
AU  - Radzioch D
FAU - Gottschalk, Marcelo
AU  - Gottschalk M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070216
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukins)
RN  - 0 (Lipopeptides)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Oligopeptides)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - DZX5IUA94D (macrophage stimulatory lipopeptide 2)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Wall/immunology
MH  - Chemokine CCL2/metabolism
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunity, Innate/drug effects/*immunology
MH  - Interleukins/metabolism
MH  - Lipopeptides
MH  - Lipopolysaccharide Receptors/genetics/metabolism
MH  - Macrophages/drug effects/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myeloid Differentiation Factor 88/genetics
MH  - Oligopeptides/pharmacology
MH  - Polysaccharides, Bacterial/*immunology/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Streptococcus suis/*immunology
MH  - Toll-Like Receptor 2/antagonists & inhibitors/genetics/*metabolism
MH  - Toll-Like Receptor 4/antagonists & inhibitors/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2007/02/20 09:00
MHDA- 2007/08/28 09:00
CRDT- 2007/02/20 09:00
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/08/28 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
AID - dxm003 [pii]
AID - 10.1093/intimm/dxm003 [doi]
PST - ppublish
SO  - Int Immunol. 2007 Apr;19(4):375-89. doi: 10.1093/intimm/dxm003. Epub 2007 Feb 16.