PMID- 17308184
OWN - NLM
STAT- MEDLINE
DCOM- 20071009
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 73
IP  - 8
DP  - 2007 Apr
TI  - Oil field souring control by nitrate-reducing Sulfurospirillum spp. that 
      outcompete sulfate-reducing bacteria for organic electron donors.
PG  - 2644-52
AB  - Nitrate injection into oil reservoirs can prevent and remediate souring, the 
      production of hydrogen sulfide by sulfate-reducing bacteria (SRB). Nitrate 
      stimulates nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB) and 
      heterotrophic nitrate-reducing bacteria (hNRB) that compete with SRB for 
      degradable oil organics. Up-flow, packed-bed bioreactors inoculated with water 
      produced from an oil field and injected with lactate, sulfate, and nitrate served 
      as sources for isolating several NRB, including Sulfurospirillum and Thauera spp. 
      The former coupled reduction of nitrate to nitrite and ammonia with oxidation of 
      either lactate (hNRB activity) or sulfide (NR-SOB activity). Souring control in a 
      bioreactor receiving 12.5 mM lactate and 6, 2, 0.75, or 0.013 mM sulfate always 
      required injection of 10 mM nitrate, irrespective of the sulfate concentration. 
      Community analysis revealed that at all but the lowest sulfate concentration 
      (0.013 mM), significant SRB were present. At 0.013 mM sulfate, direct 
      hNRB-mediated oxidation of lactate by nitrate appeared to be the dominant 
      mechanism. The absence of significant SRB indicated that sulfur cycling does not 
      occur at such low sulfate concentrations. The metabolically versatile 
      Sulfurospirillum spp. were dominant when nitrate was present in the bioreactor. 
      Analysis of cocultures of Desulfovibrio sp. strain Lac3, Lac6, or Lac15 and 
      Sulfurospirillum sp. strain KW indicated its hNRB activity and ability to produce 
      inhibitory concentrations of nitrite to be key factors for it to successfully 
      outcompete oil field SRB.
FAU - Hubert, Casey
AU  - Hubert C
AD  - Department of Biological Sciences, University of Calgary, Calgary, Alberta, 
      Canada.
FAU - Voordouw, Gerrit
AU  - Voordouw G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070216
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Organic Chemicals)
RN  - 0 (Petroleum)
RN  - 0 (Sulfates)
RN  - 0 (Sulfides)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 7664-41-7 (Ammonia)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Ammonia/metabolism
MH  - Antibiosis/*physiology
MH  - Desulfovibrio/drug effects/growth & development/metabolism
MH  - Environmental Restoration and Remediation/*methods
MH  - Epsilonproteobacteria/*growth & development/isolation & purification/metabolism
MH  - Hydrogen Sulfide/*antagonists & inhibitors
MH  - Lactic Acid/metabolism
MH  - Nitrates/*metabolism
MH  - Nitrites/metabolism
MH  - Organic Chemicals/metabolism
MH  - Oxidation-Reduction
MH  - *Petroleum/metabolism
MH  - *Soil Microbiology
MH  - Sulfates/metabolism
MH  - Sulfides/metabolism
MH  - Thauera/isolation & purification
PMC - PMC1855586
EDAT- 2007/02/20 09:00
MHDA- 2007/10/10 09:00
PMCR- 2007/08/01
CRDT- 2007/02/20 09:00
PHST- 2007/02/20 09:00 [pubmed]
PHST- 2007/10/10 09:00 [medline]
PHST- 2007/02/20 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - AEM.02332-06 [pii]
AID - 2332-06 [pii]
AID - 10.1128/AEM.02332-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2007 Apr;73(8):2644-52. doi: 10.1128/AEM.02332-06. Epub 
      2007 Feb 16.