PMID- 17309294
OWN - NLM
STAT- MEDLINE
DCOM- 20070711
LR  - 20181201
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 8
IP  - 3
DP  - 2007 Mar
TI  - Translocation of poly(ethylene glycol-co-hexadecyl)cyanoacrylate nanoparticles 
      into rat brain endothelial cells: role of apolipoproteins in receptor-mediated 
      endocytosis.
PG  - 793-9
AB  - Previous in vivo observations in rats have shown that poly(ethylene glycol) 
      polyhexadecylcyanoacrylate (PEG-PHDCA) nanoparticles could translocate into the 
      brain after intravenous injection, which polyhexadecylcyanoacrylate (PHDCA) 
      nanoparticles did not. Through the detailed analysis of the plasma protein 
      adsorption onto the surface of PEG-PHDCA nanoparticles, the present study aimed 
      at clarifying the mechanism by which nanoparticles could penetrate into rat brain 
      endothelial cells (RBEC). Two-dimensional polyacrylamide gel electrophoresis and 
      Western blotting revealed that, after incubation with rat serum, apolipoprotein E 
      (ApoE) adsorbed more onto PEG-PHDCA than on PHDCA nanoparticles. Adsorption of 
      apolipoprotein B-100 (ApoB-100) onto PEG-PHDCA nanoparticles was demonstrated by 
      capillary electrophoresis experiments. Moreover, only when ApoE or ApoB-100 were 
      preadsorbed onto PEG-PHDCA nanoparticles, nanoparticles were found to be more 
      efficient than control nanoparticles for penetrating into RBEC, suggesting the 
      involvement of a low density lipoprotein receptor in this process. Thus, these 
      data clearly demonstrate the involvement of apolipoproteins in the brain 
      transport of PEG-PHDCA nanoparticles, which may open interesting prospects for 
      brain drug delivery.
FAU - Kim, Hyun R
AU  - Kim HR
AD  - Laboratory of Biopharmacy and Pharmaceutical Technology, UMR 8612, CNRS, 
      University of Paris-Sud, F-92296 Chatenay-Malabry, France.
FAU - Andrieux, Karine
AU  - Andrieux K
FAU - Gil, Sophie
AU  - Gil S
FAU - Taverna, Myriam
AU  - Taverna M
FAU - Chacun, Helene
AU  - Chacun H
FAU - Desmaele, Didier
AU  - Desmaele D
FAU - Taran, Frederic
AU  - Taran F
FAU - Georgin, Dominique
AU  - Georgin D
FAU - Couvreur, Patrick
AU  - Couvreur P
LA  - eng
PT  - Journal Article
DEP - 20070220
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Acrylates)
RN  - 0 (Apolipoproteins)
RN  - 0 (Cyanoacrylates)
RN  - 0 (polyethyleneglycol polyhexadecylylcyanoacrylate copolymer)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Acrylates/chemistry
MH  - Adsorption
MH  - Animals
MH  - Apolipoproteins/*chemistry
MH  - Biological Transport
MH  - Brain/*metabolism
MH  - Cyanoacrylates/*chemistry
MH  - Drug Delivery Systems
MH  - Electrophoresis, Capillary
MH  - *Endocytosis
MH  - Endothelial Cells/*cytology
MH  - Nanoparticles/*chemistry
MH  - Polyethylene Glycols/*chemistry
MH  - Rats
MH  - Silver Staining
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2007/02/21 09:00
MHDA- 2007/07/12 09:00
CRDT- 2007/02/21 09:00
PHST- 2007/02/21 09:00 [pubmed]
PHST- 2007/07/12 09:00 [medline]
PHST- 2007/02/21 09:00 [entrez]
AID - 10.1021/bm060711a [doi]
PST - ppublish
SO  - Biomacromolecules. 2007 Mar;8(3):793-9. doi: 10.1021/bm060711a. Epub 2007 Feb 20.