PMID- 1731111
OWN - NLM
STAT- MEDLINE
DCOM- 19920214
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 66
IP  - 2
DP  - 1992 Feb
TI  - Tightly bound zinc in human immunodeficiency virus type 1, human T-cell leukemia 
      virus type I, and other retroviruses.
PG  - 840-7
AB  - Human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type 
      I (HTLV-I) were purified by sucrose density gradient centrifugation in the 
      presence of 1 mM EDTA. Pelleted gradient fractions were analyzed for total 
      protein, total Gag capsid protein, and total zinc. Zinc was found to copurify and 
      concentrate with the virus particles. Through successive cycles of resuspending 
      in buffer containing EDTA and repelleting, the zinc content remained constant at 
      about 1.7 mol of zinc per mol of Gag protein. Proteins from purified virus (HIV-1 
      and HTLV-I) were separated by sodium dodecyl sulfate-polyacrylamide gel 
      electrophoresis, blotted to polyvinylidene fluoride paper, and probed with 
      65ZnCl2. Viral nucleocapsid (NC) proteins (HIV-1 p7NC and HTLV-I p15NC) bound 
      65Zn2+. Other retroviruses, including simian immunodeficiency virus, equine 
      infectious anemia virus, bovine leukemia virus, Moloney murine leukemia virus, 
      mouse mammary tumor virus, and Mason-Pfizer monkey virus, were found to contain 
      amounts of zinc per milligram of total protein similar to those found in HIV-1 
      and HTLV-I. Collectively, these data support the hypothesis that retroviral NC 
      proteins function as zinc finger proteins in mature viruses.
FAU - Bess, J W Jr
AU  - Bess JW Jr
AD  - AIDS Vaccine Program, National Cancer Institute-Frederick Cancer Research and 
      Development Center, Maryland 21702-1201.
FAU - Powell, P J
AU  - Powell PJ
FAU - Issaq, H J
AU  - Issaq HJ
FAU - Schumack, L J
AU  - Schumack LJ
FAU - Grimes, M K
AU  - Grimes MK
FAU - Henderson, L E
AU  - Henderson LE
FAU - Arthur, L O
AU  - Arthur LO
LA  - eng
GR  - N01-CO-74102/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Gene Products, gag)
RN  - 0 (Viral Proteins)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Capsid/chemistry/isolation & purification
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - Gene Products, gag/chemistry/isolation & purification
MH  - HIV-1/*chemistry
MH  - Human T-lymphotropic virus 1/*chemistry
MH  - Humans
MH  - Molecular Weight
MH  - Retroviridae/*chemistry/isolation & purification
MH  - Spectrophotometry, Atomic
MH  - Viral Proteins/*chemistry/isolation & purification
MH  - Zinc/*analysis
PMC - PMC240784
EDAT- 1992/02/11 19:15
MHDA- 2001/03/28 10:01
PMCR- 1992/02/01
CRDT- 1992/02/11 19:15
PHST- 1992/02/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1992/02/11 19:15 [entrez]
PHST- 1992/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.66.2.840-847.1992 [doi]
PST - ppublish
SO  - J Virol. 1992 Feb;66(2):840-7. doi: 10.1128/JVI.66.2.840-847.1992.