PMID- 17311318
OWN - NLM
STAT- MEDLINE
DCOM- 20070426
LR  - 20220310
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 501
IP  - 6
DP  - 2007 Apr 20
TI  - Rat retinal microglial cells under normal conditions, after optic nerve section, 
      and after optic nerve section and intravitreal injection of trophic factors or 
      macrophage inhibitory factor.
PG  - 866-78
AB  - Retinal microglial cells may have a role in both degeneration and neuroprotection 
      of retinal ganglion cells (RGC) after optic nerve (ON) section. We have used 
      NDPase enzymohistochemistry to label adult rat retinal microglial cells and have 
      studied these cells under normal conditions, after left ON section, and after 
      left ON section and eye puncture or intravitreal injection of different 
      substances: vehicle, brain-derived neurotrophic factor (BDNF), nerve growth 
      factor (NGF), neurotrophin 3 (NT3), or macrophage inhibitory factor (MIF). 
      Resident microglial cells are present in four layers in the adult rat retina: the 
      nerve fiber layer (NFL), ganglion cell layer (GCL), inner plexiform layer (IPL), 
      and outer plexiform layer (OPL). Left ON section induces microglial activation in 
      the ipsilateral and contralateral retina as manifested by stronger staining 
      intensity in both retinas and increased microglial cell densities in the NFL, 
      IPL, and GCL of the ipsilateral retina. Left ON section followed by left eye 
      puncture or intravitreal injection increases microglial cell density in both 
      retinas and induces changes in the microglial cells of the ipsilateral retina 
      that vary depending on the substance injected: BDNF injections delay microglial 
      activation, possibly through retinal ganglion cell neuroprotection, whereas NT3 
      partially inhibits microglial activation in the NFL; MIF injections have no clear 
      effects on microglial activation. In conclusion, retinal microglial cells become 
      activated after an ON section and react more intensely when the eye is also 
      punctured or injected, and this response may be altered by using neurotrophic 
      factors, although the effects of MIF are less clear.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Sobrado-Calvo, Paloma
AU  - Sobrado-Calvo P
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, 30100 
      Espinardo, Murcia, Spain.
FAU - Vidal-Sanz, Manuel
AU  - Vidal-Sanz M
FAU - Villegas-Perez, Maria P
AU  - Villegas-Perez MP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/immunology/physiology
MH  - Macrophage Migration-Inhibitory Factors/immunology/*physiology
MH  - Microglia/cytology/*immunology
MH  - Nerve Growth Factor/immunology/physiology
MH  - Nerve Growth Factors/immunology/*physiology
MH  - Neurotrophin 3/immunology/physiology
MH  - Optic Nerve/immunology
MH  - Optic Nerve Injuries/*immunology/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/*cytology/immunology
MH  - Vitreous Body/immunology/metabolism
EDAT- 2007/02/22 09:00
MHDA- 2007/04/27 09:00
CRDT- 2007/02/22 09:00
PHST- 2007/02/22 09:00 [pubmed]
PHST- 2007/04/27 09:00 [medline]
PHST- 2007/02/22 09:00 [entrez]
AID - 10.1002/cne.21279 [doi]
PST - ppublish
SO  - J Comp Neurol. 2007 Apr 20;501(6):866-78. doi: 10.1002/cne.21279.