PMID- 17311319
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20071115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 46
IP  - 5
DP  - 2007 May
TI  - Molecular cytogenetic characterization of TCF3 (E2A)/19p13.3 rearrangements in 
      B-cell precursor acute lymphoblastic leukemia.
PG  - 478-86
AB  - The t(1;19)(q23;p13.3) is one of the most common chromosomal abnormalities in 
      B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and usually gives rise to 
      the TCF3-PBX1 fusion gene. Additional rare, and sometimes cytogenetically 
      cryptic, translocations involving the TCF3 gene have also been described. Using a 
      dual color split-signal fluorescence in situ hybridization (FISH) probe, we have 
      investigated the involvement of this gene in a series of BCP-ALLs harboring 19p13 
      translocations, as well as an unselected patient cohort. The TCF3 gene was shown 
      to be involved in the majority of cases with a cytogenetically visible t(1;19) 
      translocation, while the remaining TCF3-negative ALLs demonstrated breakpoint 
      heterogeneity. Although most "other" 19p13 translocations did not produce a 
      split-signal FISH pattern, a novel t(13;19)(q14;p13) involving TCF3 was 
      discovered. A prospective screen of 161 children with BCP-ALL revealed a cryptic 
      t(12;19)(p13;p13), another novel TCF3 rearrangement, and a series of patients 
      with submicroscopic deletions of TCF3. These results demonstrate the utility of a 
      split-signal FISH strategy in confirming the involvement of the TCF3 gene in 
      19p13 rearrangements and in identifying novel and cryptic TCF3 translocations. In 
      addition to its role as a fusion partner gene, we propose that TCF3 can also act 
      as a tumor suppressor gene in BCP-ALL.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Barber, Kerry E
AU  - Barber KE
AD  - Leukaemia Research Cytogenetics Group, Cancer Sciences Division, University of 
      Southampton, Southampton, UK.
FAU - Harrison, Christine J
AU  - Harrison CJ
FAU - Broadfield, Zoe J
AU  - Broadfield ZJ
FAU - Stewart, Adam R M
AU  - Stewart AR
FAU - Wright, Sarah L
AU  - Wright SL
FAU - Martineau, Mary
AU  - Martineau M
FAU - Strefford, Jon C
AU  - Strefford JC
FAU - Moorman, Anthony V
AU  - Moorman AV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (TCF3 protein, human)
SB  - IM
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics
MH  - Burkitt Lymphoma/*genetics/pathology
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 19
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Precancerous Conditions/genetics/pathology
MH  - Sequence Deletion
MH  - *Translocation, Genetic
EDAT- 2007/02/22 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/02/22 09:00
PHST- 2007/02/22 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/02/22 09:00 [entrez]
AID - 10.1002/gcc.20431 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2007 May;46(5):478-86. doi: 10.1002/gcc.20431.