PMID- 17311354
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20191210
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 98
IP  - 3
DP  - 2007 Oct 15
TI  - Using respirometric techniques and fluorescent in situ hybridization to evaluate 
      the heterotrophic active biomass in activated sludge.
PG  - 561-8
AB  - The separation and accurate quantification of active biomass components in 
      activated sludge is of paramount importance in models, used for the management 
      and design of waste water (WW) treatment plants. Accurate estimates of microbial 
      population concentrations and the direct, in situ determination of kinetic 
      parameters could improve the calibration and validation of existing models of 
      biological nutrient removal activated sludge systems. The aim of this study was 
      to obtain correlations between heterotrophic active biomass (Z(BH)) 
      concentrations predicted by mathematical models and quantitative information 
      obtained by Fluorescent in situ hybridizations (FISH). Respirometric batch test 
      were applied to mixed liquors drawn from a well-defined parent anoxic/aerobic 
      activated sludge system to quantify the Z(BH) concentrations. Similarly 
      fluorescent labeled, 16S rRNA-targeted oligonucleotide probes specific for 
      ammonia and nitrite oxidizers were used in combination with DAPI staining to 
      validate the Z(BH) active biomass component in activate sludge respirometric 
      batch tests. For the direct enumeration and simultaneous in situ analysis of the 
      distribution of nitrifying bacteria, in situ hybridization with oligonucleotide 
      probes were used. Probes (NSO 1225, NSR 1156, and NIT3) were used to target the 
      nitrifiers and the universal probe (EUB MIX) was used to target all Eubacteria. 
      Deducting the lithoautotrophic population from the total bacteria population 
      revealed the Z(BH) population. A conversion factor of 8.49 x 10(-11) mg VSS/cell 
      was applied to express the Z(BH) in terms of COD concentration. Z(BH) values 
      obtained by molecular probing correlated closely with values obtained from the 
      modified batch test. However, the trend of consistently poor correspondence of 
      measured and theoretical concentrations were evident. Therefore, the focus of 
      this study was to investigate alternative technology, such as FISH to validate or 
      replace kinetic parameters which are invariably incorporated into models.
CI  - Copyright 2007 Wiley Periodicals, Inc.
FAU - Ismail, A
AU  - Ismail A
AD  - Centre for Water and Wastewater Technology, Department of Biotechnology, Durban 
      Institute of Technology, PO Box 1334 Durban 4000, South Africa. arshadi@dit.ac.za
FAU - Wentzel, M C
AU  - Wentzel MC
FAU - Bux, F
AU  - Bux F
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Sewage)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Colony Count, Microbial/*methods
MH  - Computer Simulation
MH  - Gram-Positive Bacteria/cytology/*isolation & purification/*physiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Models, Biological
MH  - Oxygen/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sewage/*microbiology
EDAT- 2007/02/22 09:00
MHDA- 2007/11/14 09:00
CRDT- 2007/02/22 09:00
PHST- 2007/02/22 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/02/22 09:00 [entrez]
AID - 10.1002/bit.21042 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2007 Oct 15;98(3):561-8. doi: 10.1002/bit.21042.