PMID- 17312130
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 5
DP  - 2007 Mar 1
TI  - Involvement of Src and Syk tyrosine kinases in HIV-1 transfer from dendritic 
      cells to CD4+ T lymphocytes.
PG  - 2862-71
AB  - Dendritic cells (DCs) are considered as key mediators of the early events in 
      HIV-1 infection at mucosal sites. Although several aspects of the complex 
      interactions between DCs and HIV-1 have been elucidated, there are still basic 
      questions that remain to be answered about DCs/HIV-1 interplay. In this study, we 
      examined the contribution of nonreceptor TKs in the known ability of DCs to 
      efficiently transfer HIV-1 to CD4(+) T cells in trans. Experiments performed with 
      specific inhibitors of Src and Syk family members indicate that these tyrosine 
      kinases (TKs) are participating to HIV-1 transfer from immature monocyte-derived 
      DCs (IM-MDDCs) to autologous CD4(+) T cells. Experiments with IM-MDDCs 
      transfected with small interfering RNAs targeting Lyn and Syk confirmed the 
      importance of these nonreceptor TKs in HIV-1 transmission. The Src- and 
      Syk-mediated effect on virus transfer was linked with infection of IM-MDDCs in 
      cis-as monitored by quantifying integrated viral DNA and de novo virus 
      production. The process of HIV-1 transmission from IM-MDDCs to CD4(+) T cells was 
      unaffected following treatment with protein kinase C and protein kinase A 
      inhibitors. These data suggest that Src and Syk TKs play a functional role in 
      productive HIV-1 infection of IM-MDDCs. Additional work is needed to facilitate 
      our comprehension of the various mechanisms underlying the exact contribution of 
      Src and Syk TKs to this phenomenon.
FAU - Gilbert, Caroline
AU  - Gilbert C
AD  - Centre de Recherche en Infectiologie, Centre Hospitalier de l'Universite Laval, 
      and Faculte de Medecine, Universite Laval, 2705 Boulevard Laurier, Quebec, 
      Canada.
FAU - Barat, Corinne
AU  - Barat C
FAU - Cantin, Rejean
AU  - Cantin R
FAU - Tremblay, Michel J
AU  - Tremblay MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology/virology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/virology
MH  - HIV Infections/immunology/*transmission
MH  - HIV-1/*immunology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/antagonists & 
      inhibitors/*immunology
MH  - Monocytes/immunology/virology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/*immunology
MH  - Proto-Oncogene Proteins pp60(c-src)/*immunology
MH  - RNA, Small Interfering/immunology/pharmacology
MH  - Syk Kinase
EDAT- 2007/02/22 09:00
MHDA- 2007/04/25 09:00
CRDT- 2007/02/22 09:00
PHST- 2007/02/22 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2007/02/22 09:00 [entrez]
AID - 178/5/2862 [pii]
AID - 10.4049/jimmunol.178.5.2862 [doi]
PST - ppublish
SO  - J Immunol. 2007 Mar 1;178(5):2862-71. doi: 10.4049/jimmunol.178.5.2862.