PMID- 17312223
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20181201
IS  - 1526-7598 (Electronic)
IS  - 0003-2999 (Linking)
VI  - 104
IP  - 3
DP  - 2007 Mar
TI  - The immunomodulatory effect of sevoflurane in endotoxin-injured alveolar 
      epithelial cells.
PG  - 638-45
AB  - BACKGROUND: Endotoxin-induced lung injury is a useful experimental system for the 
      characterization of immunopathologic mechanisms in acute lung injury. Although 
      alveolar epithelial cells (AEC) are directly exposed to volatile anesthetics, 
      there is limited information about the effect of anesthetics on these cells. In 
      this study we investigated the effect of pretreatment with the inhaled anesthetic 
      sevoflurane on lipopolysaccharide (LPS)-injured AEC. METHODS: AEC were incubated 
      with 1.1 vol % sevoflurane for 0.5 h, followed by LPS stimulation for 5 h. 
      Expression of monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory 
      protein-1beta (MIP-1beta), macrophage inflammatory protein-2 (MIP-2), 
      cytokine-induced neutrophil chemoattractant-1 (CINC-1), and intercellular 
      adhesion molecule-1 (ICAM-1) was analyzed. In addition, functional tests were 
      performed through chemotaxis and adherence assays to underline the biological 
      relevance of the findings. RESULTS: Exposure of AEC to sevoflurane resulted in a 
      50% downregulation of MCP-1 protein in the sevoflurane-LPS group when compared 
      with non-sevoflurane- LPS cells (P < 0.05). MIP-1beta concentration in 
      LPS-stimulated cells decreased by 32% with sevoflurane (P < 0.05), MIP-2 by 29% 
      (P < 0.05), and CINC-1 by 20% (P < 0.05). ICAM-1 protein expression was 
      attenuated by 36% (P < 0.05). This inhibition caused substantial changes in the 
      inflammatory response of neutrophils. 33% less chemotactic activity was seen in 
      sevoflurane-treated LPS cells (P < 0.001) as well as 47% decreased adhesion of 
      neutrophils to AEC (P < 0.001). CONCLUSIONS: This study shows that sevoflurane 
      alters the LPS-induced inflammatory response, not only with respect to the 
      expression pattern of inflammatory mediators, but also regarding the biological 
      consequences with less accumulation of effector cells such as neutrophils.
FAU - Suter, Dominik
AU  - Suter D
AD  - Institute of Anesthesiology, University of Zurich, Zurich, Switzerland.
FAU - Spahn, Donat R
AU  - Spahn DR
FAU - Blumenthal, Stephan
AU  - Blumenthal S
FAU - Reyes, Livia
AU  - Reyes L
FAU - Booy, Christa
AU  - Booy C
FAU - Z'graggen, Birgit Roth
AU  - Z'graggen BR
FAU - Beck-Schimmer, Beatrice
AU  - Beck-Schimmer B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Anesthetics)
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl1 protein, rat)
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Methyl Ethers)
RN  - 0 (Monokines)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 38LVP0K73A (Sevoflurane)
SB  - IM
MH  - Anesthetics/pharmacology
MH  - Anesthetics, Inhalation/pharmacology
MH  - Animals
MH  - Cell Adhesion
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL4
MH  - Chemokine CXCL1
MH  - Chemokine CXCL2
MH  - Chemokines, CXC/metabolism
MH  - Endotoxins/*pharmacology
MH  - Epithelium/*drug effects
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Lipopolysaccharides/metabolism/pharmacology
MH  - Lung/cytology
MH  - Macrophage Inflammatory Proteins/metabolism
MH  - Methyl Ethers/*pharmacology
MH  - Monokines/metabolism
MH  - Neutrophils/drug effects/metabolism
MH  - Pulmonary Alveoli/*drug effects
MH  - Rats
MH  - Sevoflurane
EDAT- 2007/02/22 09:00
MHDA- 2007/03/28 09:00
CRDT- 2007/02/22 09:00
PHST- 2007/02/22 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2007/02/22 09:00 [entrez]
AID - 104/3/638 [pii]
AID - 10.1213/01.ane.0000255046.06058.58 [doi]
PST - ppublish
SO  - Anesth Analg. 2007 Mar;104(3):638-45. doi: 10.1213/01.ane.0000255046.06058.58.