PMID- 1731336
OWN - NLM
STAT- MEDLINE
DCOM- 19920218
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 2
DP  - 1992 Jan 15
TI  - Differential regulation of mRNAs for nerve growth factor, brain-derived 
      neurotrophic factor, and neurotrophin 3 in the adult rat brain following cerebral 
      ischemia and hypoglycemic coma.
PG  - 648-52
AB  - In situ hybridization was used to study expression of mRNAs for members of the 
      nerve growth factor (NGF) family in the rat brain after 2 and 10 min of forebrain 
      ischemia and 1 and 30 min of insulin-induced hypoglycemic coma. Two hours after 
      the ischemic insults, the level of brain-derived neurotrophic factor (BDNF) mRNA 
      was markedly increased in the granule cells of the dentate gyrus, and at 24 h it 
      was still significantly elevated. NGF mRNA showed a pronounced increase 4 h after 
      2 min of ischemia but had returned to a control level at 24 h. Both 2 and 10 min 
      of ischemia caused a clear reduction of the level of mRNA for neurotrophin 3 
      (NT-3) in the dentate granule cells and in regions CA2 and medial CA1 of the 
      hippocampus 2 and 4 h after the insults. The increase of BDNF mRNA could be 
      partially blocked by the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid 
      (AMPA) receptor antagonist NBQX but was not influenced by the 
      N-methyl-D-aspartate (NMDA) receptor antagonist MK-801. Both NBQX and MK-801 
      attenuated the decrease of NT-3 mRNA after ischemia. One and 30 min of 
      hypoglycemic coma also induced marked increases in BDNF and NGF mRNA in dentate 
      granule cells with maximal levels at 2 h. If the changes of mRNA expression lead 
      to alterations in the relative availability of neurotrophic factors, this could 
      influence functional outcome and neuronal necrosis following ischemic and 
      hypoglycemic insults.
FAU - Lindvall, O
AU  - Lindvall O
AD  - Department of Neurology, University Hospital, Lund, Sweden.
FAU - Ernfors, P
AU  - Ernfors P
FAU - Bengzon, J
AU  - Bengzon J
FAU - Kokaia, Z
AU  - Kokaia Z
FAU - Smith, M L
AU  - Smith ML
FAU - Siesjo, B K
AU  - Siesjo BK
FAU - Persson, H
AU  - Persson H
LA  - eng
GR  - AG 04418/AG/NIA NIH HHS/United States
GR  - NS 09199/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Quinoxalines)
RN  - 0 (RNA, Messenger)
RN  - 118876-58-7 (2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
SB  - IM
MH  - Animals
MH  - Brain/*physiopathology
MH  - Brain-Derived Neurotrophic Factor
MH  - Dizocilpine Maleate/pharmacology
MH  - Gene Expression
MH  - Hippocampus/physiopathology
MH  - Insulin Coma/*physiopathology
MH  - Ischemic Attack, Transient/*physiopathology
MH  - Male
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurotrophin 3
MH  - Nucleic Acid Hybridization
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - Quinoxalines/pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Time Factors
PMC - PMC48296
EDAT- 1992/01/15 00:00
MHDA- 1992/01/15 00:01
PMCR- 1992/07/15
CRDT- 1992/01/15 00:00
PHST- 1992/01/15 00:00 [pubmed]
PHST- 1992/01/15 00:01 [medline]
PHST- 1992/01/15 00:00 [entrez]
PHST- 1992/07/15 00:00 [pmc-release]
AID - 10.1073/pnas.89.2.648 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):648-52. doi: 10.1073/pnas.89.2.648.