PMID- 17313466
OWN - NLM
STAT- MEDLINE
DCOM- 20071108
LR  - 20070222
IS  - 1398-2273 (Print)
IS  - 1398-2273 (Linking)
VI  - 9
IP  - 1
DP  - 2007 Mar
TI  - Analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay, the 
      amplicor CMV test, and a semi-quantitative polymerase chain reaction test after 
      allogeneic marrow transplantation.
PG  - 16-21
AB  - A pp65 antigenemia assay for polymorphonuclear leukocytes (PMNLs) (CINAkit Rapid 
      Antigenemia), and a qualitative polymerase chain reaction (PCR) test for plasma 
      'PCR-P qual' (Amplicor cytomegalovirus [CMV] test) were performed for 126 samples 
      (blood and plasma) obtained from 18 bone marrow transplant patients, over a 
      9-month surveillance period. Among those samples, 92 were assayed with a 
      semi-quantitative PCR test for PMNLs 'PCR-L quant.' The number of samples with a 
      positive CMV test for antigenemia and PCR-P qual assays was 20.63% and 12.7%, 
      respectively, whereas the PCR-L quant assay was positive in 48 of the 92 samples 
      assayed (52.17%). The rates of concordance of the results of PCR-P qual and 
      antigenemia, PCR-P qual and PCR-L quant, antigenemia and PCR-L quant were 92%, 
      65.2% and 66.8%, respectively. The analysis of the results for the 92 specimens 
      tested by all 3 methods showed a rate of concordance of 63% among all methods. 
      Good agreement (kappa=0.72) was found only between pp65 Ag and PCR-P qual assays. 
      Clinical disease correlates with an antigenemia high viral load. Three patients 
      had CMV disease despite preemptive therapy, and all of them had 
      graft-versus-host-disease (GVHD). PMNLs-based assays are more efficient in 
      monitoring CMV reactivation, but for high-risk patients with GVHD, more sensitive 
      assays (real-time PCR) must be done.
FAU - Ksouri, H
AU  - Ksouri H
AD  - Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, 
      Tunisia. ksourih_2000@yahoo.fr
FAU - Eljed, H
AU  - Eljed H
FAU - Greco, A
AU  - Greco A
FAU - Lakhal, A
AU  - Lakhal A
FAU - Torjman, L
AU  - Torjman L
FAU - Abdelkefi, A
AU  - Abdelkefi A
FAU - Ben Othmen, T
AU  - Ben Othmen T
FAU - Ladeb, S
AU  - Ladeb S
FAU - Slim, A
AU  - Slim A
FAU - Zouari, B
AU  - Zouari B
FAU - Abdeladhim, A
AU  - Abdeladhim A
FAU - Ben Hassen, A
AU  - Ben Hassen A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Denmark
TA  - Transpl Infect Dis
JT  - Transplant infectious disease : an official journal of the Transplantation 
      Society
JID - 100883688
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Phosphoproteins)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Viral/blood
MH  - Bone Marrow Transplantation/adverse effects
MH  - Child
MH  - Cytomegalovirus/genetics/immunology/*isolation & purification
MH  - Cytomegalovirus Infections/blood/*diagnosis/etiology
MH  - DNA, Viral/blood
MH  - Female
MH  - Graft vs Host Disease/etiology
MH  - Granulocytes/immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Phosphoproteins/*blood
MH  - Polymerase Chain Reaction
MH  - *Reagent Kits, Diagnostic
MH  - Sensitivity and Specificity
MH  - Transplantation Conditioning/adverse effects
MH  - Viral Load
MH  - Viral Matrix Proteins/*blood
MH  - Viremia/blood/diagnosis
EDAT- 2007/02/23 09:00
MHDA- 2007/11/09 09:00
CRDT- 2007/02/23 09:00
PHST- 2007/02/23 09:00 [pubmed]
PHST- 2007/11/09 09:00 [medline]
PHST- 2007/02/23 09:00 [entrez]
AID - TID171 [pii]
AID - 10.1111/j.1399-3062.2006.00171.x [doi]
PST - ppublish
SO  - Transpl Infect Dis. 2007 Mar;9(1):16-21. doi: 10.1111/j.1399-3062.2006.00171.x.