PMID- 17313586
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20070222
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 59
IP  - 2
DP  - 2007 Feb
TI  - Comparative phylogenetic analysis of microbial communities in pristine and 
      hydrocarbon-contaminated Alpine soils.
PG  - 466-75
AB  - A molecular characterization of pristine and petroleum hydrocarbon-contaminated 
      Alpine soils sampled in Tyrol (Austria) was performed. To identify predominant 
      bacteria, PCR-amplified 16S rRNA gene fragments from five pristine and nine 
      contaminated soils were analysed using denaturing gradient gel electrophoresis 
      (DGGE). Sequencing and phylogenetic analyses demonstrated that the majority of 
      the DGGE bands represented bacteria in the Actinobacteria and Proteobacteria 
      phyla: 18 and 73%, respectively, in pristine soils, compared with 20 and 76%, 
      respectively, in contaminated soils. A different distribution pattern of 
      bacterial classes in the Proteobacteria was observed between pristine and 
      contaminated soils. The relative proportion of microorganisms belonging to the 
      Alphaproteobacteria was larger in pristine (46%) than in contaminated (24%) 
      soils, while Betaproteobacteria and Gammaproteobacteria were detected only in the 
      hydrocarbon-contaminated soils. This result compared favourably with earlier work 
      in which hydrocarbon-degradation genotypes, largely pseudomonads and 
      Acinetobacter, belonging to the Gammaproteobacteria, were enriched following oil 
      hydrocarbon contamination. In contrast, members of the Actinobacteria phylum, 
      represented by Rhodococcus and Mycobacterium, were found in pristine soils where 
      contamination events had not occurred. The results demonstrate a significant 
      shift in the microbial community structure in Alpine soils following 
      contamination. Furthermore, more potentially novel phylotypes were found in the 
      pristine soils than in the contaminated soils.
FAU - Labbe, Diane
AU  - Labbe D
AD  - NRC-Biotechnology Research Institute, Montreal, Canada.
FAU - Margesin, Rosa
AU  - Margesin R
FAU - Schinner, Franz
AU  - Schinner F
FAU - Whyte, Lyle G
AU  - Whyte LG
FAU - Greer, Charles W
AU  - Greer CW
LA  - eng
SI  - GENBANK/DQ525802
SI  - GENBANK/DQ525803
SI  - GENBANK/DQ525804
SI  - GENBANK/DQ525805
SI  - GENBANK/DQ525806
SI  - GENBANK/DQ525807
SI  - GENBANK/DQ525808
SI  - GENBANK/DQ525809
SI  - GENBANK/DQ525810
SI  - GENBANK/DQ525811
SI  - GENBANK/DQ525812
SI  - GENBANK/DQ525813
SI  - GENBANK/DQ525814
SI  - GENBANK/DQ525815
SI  - GENBANK/DQ525816
SI  - GENBANK/DQ525817
SI  - GENBANK/DQ525818
SI  - GENBANK/DQ525819
SI  - GENBANK/DQ525820
SI  - GENBANK/DQ525821
SI  - GENBANK/DQ525822
SI  - GENBANK/DQ525823
SI  - GENBANK/DQ525824
SI  - GENBANK/DQ525825
SI  - GENBANK/DQ525826
SI  - GENBANK/DQ525827
SI  - GENBANK/DQ525828
SI  - GENBANK/DQ525829
SI  - GENBANK/DQ525830
SI  - GENBANK/DQ525831
SI  - GENBANK/DQ525832
SI  - GENBANK/DQ525833
SI  - GENBANK/DQ525834
SI  - GENBANK/DQ525835
SI  - GENBANK/DQ525836
SI  - GENBANK/DQ525837
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (Hydrocarbons)
RN  - 0 (Petroleum)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Actinobacteria/*classification/genetics
MH  - Altitude
MH  - Austria
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Hydrocarbons/*metabolism
MH  - Molecular Sequence Data
MH  - Petroleum/metabolism
MH  - *Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Proteobacteria/*classification/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Soil Microbiology
MH  - Soil Pollutants/*metabolism
EDAT- 2007/02/23 09:00
MHDA- 2007/03/28 09:00
CRDT- 2007/02/23 09:00
PHST- 2007/02/23 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2007/02/23 09:00 [entrez]
AID - FEM250 [pii]
AID - 10.1111/j.1574-6941.2006.00250.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2007 Feb;59(2):466-75. doi: 
      10.1111/j.1574-6941.2006.00250.x.