PMID- 17316568
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 355
IP  - 3
DP  - 2007 Apr 13
TI  - A critical role for p38 map kinase in NF-kappaB signaling during intermittent 
      hypoxia/reoxygenation.
PG  - 728-33
AB  - NF-kappaB-dependent inflammatory gene expression is induced by intermittent 
      hypoxia/reoxygenation (IHR), an event that we have hypothesized may contribute to 
      the cardiovascular pathophysiology associated with obstructive sleep apnoea 
      syndrome (OSAS). Here, we have investigated the cellular signaling mechanisms 
      involved. Using an established endothelial cell in vitro model, we confirm a 
      selective activation of the pro-inflammatory transcription factor NF-kappaB over 
      the adaptive hypoxia-inducible factor-1 (HIF-1) in IHR-stimulated bovine aortic 
      endothelial cells. IHR activates the I-kappaB kinase (IKK) complex, leading to 
      phosphorylation/degradation of I-kappaB alpha. IHR activates p38 MAPK and 
      pharmacological inhibition of p38 (using SB 203580, 10 microM) abolishes 
      NF-kappaB activation by IHR. Furthermore, depletion of p38 using siRNA 
      significantly reduces IHR-induced NF-kappaB activity. Thus, IHR activates 
      NF-kappaB in an IKK-dependent manner signaled at least in part via activation of 
      p38 MAPK.
FAU - Ryan, Silke
AU  - Ryan S
AD  - School of Medicine and Medical Science, UCD Conway Institute, University College 
      Dublin, Belfield, Dublin 4, Ireland.
FAU - McNicholas, Walter T
AU  - McNicholas WT
FAU - Taylor, Cormac T
AU  - Taylor CT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070209
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - HeLa Cells
MH  - Humans
MH  - Hypoxia/*enzymology
MH  - Hypoxia-Inducible Factor 1/genetics/*metabolism
MH  - I-kappa B Kinase/metabolism
MH  - NF-kappa B/*metabolism
MH  - Oxygen/metabolism
MH  - Phosphorylation
MH  - RNA, Small Interfering/pharmacology
MH  - Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & 
      inhibitors/genetics/*physiology
EDAT- 2007/02/24 09:00
MHDA- 2007/05/09 09:00
CRDT- 2007/02/24 09:00
PHST- 2007/02/01 00:00 [received]
PHST- 2007/02/03 00:00 [accepted]
PHST- 2007/02/24 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2007/02/24 09:00 [entrez]
AID - S0006-291X(07)00286-0 [pii]
AID - 10.1016/j.bbrc.2007.02.015 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Apr 13;355(3):728-33. doi: 
      10.1016/j.bbrc.2007.02.015. Epub 2007 Feb 9.