PMID- 17316787
OWN - NLM
STAT- MEDLINE
DCOM- 20070718
LR  - 20191210
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 28
IP  - 16
DP  - 2007 Jun
TI  - The differential influence of colocalized and segregated dual protein signals on 
      neurite outgrowth on surfaces.
PG  - 2590-602
AB  - We present an in vitro micropatterning approach in which the density and spatial 
      presentation of two separate protein layers can be independently controlled to 
      form cell stripe assays through (1) the simultaneous application of microcontact 
      printing (microCP) and microfluidic network (microFN) patterning to generate 
      alternating stripes of pure single protein layers or (2) through microCP onto a 
      pre-adsorbed homogeneous protein layer to generate alternating single and dual 
      protein stripes. This approach enabled the creation of choice boundaries in which 
      protein-protein interactions were limited and the effects of spatially segregated 
      or colocalized dual protein signals on model primary neuronal behavior could be 
      readily interrogated and compared on both glass and tissue culture polystyrene 
      substrates. Dorsal root ganglion (DRG) cell body attachment was dictated largely 
      by non-specific cell adhesion interactions and interactions between the guidance 
      molecules laminin and aggrecan were insufficient to explain aggrecan inhibition 
      on neurite outgrowth. The presentation of a specific laminin epitope stabilized 
      by interactions with aggrecan and destabilized by microCP was a strong predictor 
      of neurite promoting activity. These observations provide evidence that aggrecan 
      is intrinsically inhibitory and that laminin-aggrecan interactions do not 
      diminish laminin growth promoting properties.
FAU - Hodgkinson, Gerald N
AU  - Hodgkinson GN
AD  - Department of Bioengineering, University of Utah, 20 S. 2030 E., Rm. 108, Salt 
      Lake City, Utah 84112, USA.
FAU - Tresco, Patrick A
AU  - Tresco PA
FAU - Hlady, Vladimir
AU  - Hlady V
LA  - eng
GR  - R01 HL084586/HL/NHLBI NIH HHS/United States
GR  - 5R01 EB000463-03/EB/NIBIB NIH HHS/United States
GR  - R01 HL084586-14A2/HL/NHLBI NIH HHS/United States
GR  - R01 NS057144/NS/NINDS NIH HHS/United States
GR  - R01 EB000463/EB/NIBIB NIH HHS/United States
GR  - R01 NS057144-01A2/NS/NINDS NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070207
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Aggrecans)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Epitopes)
RN  - 0 (Laminin)
SB  - IM
MH  - Aggrecans/*metabolism
MH  - Animals
MH  - Cell Adhesion/physiology
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/metabolism
MH  - Epitopes
MH  - Ganglia, Spinal/cytology
MH  - Laminin/*metabolism/*physiology
MH  - Materials Testing
MH  - Microfluidics
MH  - Neurites/*metabolism
MH  - Rats
MH  - Signal Transduction/*physiology
MH  - Surface Properties
PMC - PMC1892536
MID - NIHMS20750
EDAT- 2007/02/24 09:00
MHDA- 2007/07/19 09:00
PMCR- 2008/06/01
CRDT- 2007/02/24 09:00
PHST- 2006/11/02 00:00 [received]
PHST- 2007/01/23 00:00 [accepted]
PHST- 2007/02/24 09:00 [pubmed]
PHST- 2007/07/19 09:00 [medline]
PHST- 2007/02/24 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - S0142-9612(07)00084-1 [pii]
AID - 10.1016/j.biomaterials.2007.01.038 [doi]
PST - ppublish
SO  - Biomaterials. 2007 Jun;28(16):2590-602. doi: 10.1016/j.biomaterials.2007.01.038. 
      Epub 2007 Feb 7.