PMID- 17316888
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20161124
IS  - 0169-5002 (Print)
IS  - 0169-5002 (Linking)
VI  - 56
IP  - 3
DP  - 2007 Jun
TI  - Identification of genes differentially expressed in human primary lung squamous 
      cell carcinoma.
PG  - 307-17
AB  - To identify differentially expressed genes in lung squamous cell carcinomas 
      (SCCs), the suppression subtractive hybridization method (SSH) was performed 
      comparing six lung tumour tissues and 10 morphologically normal bronchial 
      epithelial tissues. A cDNA library consisting of 220 upregulated genes in tumour 
      tissue was established and named as LSCC (lung squamous cell carcinoma). Of them, 
      six were tested using semi-quantitative reverse transcription-PCR on 27 pairs of 
      tumour tissue and normal lung tissue. Differential expression was confirmed in 
      five of these six genes, including IGFBP5, SQLE, RAP2B, CLDN1, and TBL1XR1. The 
      elevated mRNA expression of RAP2B, CLDN1 and TBL1XR1, three genes located on 
      chromosome 3q, were further validated in 64.3% (18/28), 82.1% (23/28), and 75.0% 
      (21/28) of lung SCC tumour tissues, respectively, by quantitative real-time 
      reverse transcription-PCR analysis. Moreover, western blot analysis showed that 
      the protein expression of TBL1XR1 was also upregulated in 53.3% (8/15) of lung 
      SCC tumour samples, as well as in five lung cancer cell lines and in one human 
      immortalized bronchial epithelial cell line. All the initial characteristics of 
      these genes were first reported in the lung SCCs. The differentially expressed 
      genes reported in this study will provide a valuable resource for understanding 
      the pathogenesis of lung SCCs and for discovery of novel diagnostic or 
      therapeutic targets.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Etiology and Carcinogenesis, Cancer Institute (Hospital), Peking 
      Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, PR 
      China.
FAU - Sun, Wenyue
AU  - Sun W
FAU - Zhang, Kaitai
AU  - Zhang K
FAU - Zheng, Hongwei
AU  - Zheng H
FAU - Ma, Ying
AU  - Ma Y
FAU - Lin, Dongmei
AU  - Lin D
FAU - Zhang, Xinyu
AU  - Zhang X
FAU - Feng, Lin
AU  - Feng L
FAU - Lei, Wendong
AU  - Lei W
FAU - Zhang, Ziqiang
AU  - Zhang Z
FAU - Guo, Suping
AU  - Guo S
FAU - Han, Naijun
AU  - Han N
FAU - Tong, Wei
AU  - Tong W
FAU - Feng, Xiaoli
AU  - Feng X
FAU - Gao, Yanning
AU  - Gao Y
FAU - Cheng, Shujun
AU  - Cheng S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070220
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CLDN1 protein, human)
RN  - 0 (Claudin-1)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 5)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Repressor Proteins)
RN  - 0 (TBL1XR1 protein, human)
RN  - EC 1.14.14.17 (Squalene Monooxygenase)
RN  - EC 3.6.1.- (RAP2B protein, human)
RN  - EC 3.6.5.2 (rap GTP-Binding Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Biopsy
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Claudin-1
MH  - Diagnosis, Differential
MH  - Epithelial Cells/pathology
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 5/genetics
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Membrane Proteins/genetics
MH  - Middle Aged
MH  - Nuclear Proteins/genetics
MH  - RNA, Neoplasm/*genetics
MH  - Receptors, Cytoplasmic and Nuclear/genetics
MH  - Repressor Proteins/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Squalene Monooxygenase/genetics
MH  - Tight Junctions
MH  - rap GTP-Binding Proteins/genetics
EDAT- 2007/02/24 09:00
MHDA- 2007/07/20 09:00
CRDT- 2007/02/24 09:00
PHST- 2006/11/14 00:00 [received]
PHST- 2007/01/12 00:00 [revised]
PHST- 2007/01/14 00:00 [accepted]
PHST- 2007/02/24 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2007/02/24 09:00 [entrez]
AID - S0169-5002(07)00067-0 [pii]
AID - 10.1016/j.lungcan.2007.01.016 [doi]
PST - ppublish
SO  - Lung Cancer. 2007 Jun;56(3):307-17. doi: 10.1016/j.lungcan.2007.01.016. Epub 2007 
      Feb 20.