PMID- 1731796
OWN - NLM
STAT- MEDLINE
DCOM- 19920218
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 182
IP  - 1
DP  - 1992 Jan 15
TI  - Identification and characterization of a new binding site for angiotensin II in 
      mouse neuroblastoma neuro-2A cells.
PG  - 388-94
AB  - Specific binding site for 125I-angiotensin II (Ang II), with unique 
      pharmacological properties uncommon to the hitherto recognized receptor subtypes, 
      was observed in mouse neuroblastoma cells (Neuro-2A). Differentiation of the 
      cells with 100 nM PGE1 resulted in a 10-fold increase in the number of Ang II 
      binding sites without changing the binding affinity (Kd value: 12.0 nM). 125I-Ang 
      II binding to membranes of differentiated Neuro-2A was inhibited by unlabeled Ang 
      II with a Ki value of 7.06 +/- 1.09 nM but not by Ang III (1 microM). Both AT1 
      antagonist, Dup753, and AT2 antagonist, PD123319, failed to inhibit 125I-Ang II 
      binding at 1 microM. 125I-Ang II binding was not affected by GTP analogs such as 
      GTP gamma S and Gpp(NH)p. These results suggest that Neuro-2A cells possess a 
      binding site for Ang II which is different from the presently known subtypes of 
      Ang II receptors, and that the number of the binding site is regulated by cell 
      differentiation.
FAU - Chaki, S
AU  - Chaki S
AD  - Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, 
      TN 37232.
FAU - Inagami, T
AU  - Inagami T
LA  - eng
GR  - HL-14192/HL/NHLBI NIH HHS/United States
GR  - HL-35323/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Receptors, Angiotensin)
RN  - 11128-99-7 (Angiotensin II)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Angiotensin II/*metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Dithiothreitol/pharmacology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Kinetics
MH  - Mice
MH  - Neuroblastoma
MH  - Receptors, Angiotensin/drug effects/isolation & purification/*metabolism
EDAT- 1992/01/15 00:00
MHDA- 1992/01/15 00:01
CRDT- 1992/01/15 00:00
PHST- 1992/01/15 00:00 [pubmed]
PHST- 1992/01/15 00:01 [medline]
PHST- 1992/01/15 00:00 [entrez]
AID - S0006-291X(05)80157-3 [pii]
AID - 10.1016/s0006-291x(05)80157-3 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1992 Jan 15;182(1):388-94. doi: 
      10.1016/s0006-291x(05)80157-3.