PMID- 17318634
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20220716
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 116
IP  - 3
DP  - 2007 Jun
TI  - Evolutionarily conserved, cell type and species-specific higher order chromatin 
      arrangements in interphase nuclei of primates.
PG  - 307-20
AB  - Several studies demonstrated a gene-density-correlated radial organization of 
      chromosome territories (CTs) in spherically shaped nuclei of human lymphocytes or 
      lymphoblastoid cells, while CT arrangements in flat-ellipsoidal nuclei of human 
      fibroblasts are affected by both gene density and chromosome size. In the present 
      study, we performed fluorescence in situ hybridization (FISH) experiments to 
      three-dimensionally preserved nuclei (3D-FISH) from human and nonhuman primate 
      cultured lymphoblastoid cells and fibroblasts. We investigated apes, Old, and New 
      World monkeys showing either evolutionarily conserved karyotypes, multiple 
      translocations, fusions, or serial fissions. Our goal was to test whether cell 
      type specific differences of higher order chromatin arrangements are 
      evolutionarily conserved in different primate lineages. Whole genome painting 
      experiments and further detailed analyses of individual chromosomes indicate a 
      gene-density-correlated higher order organization of chromatin in lymphoblastoid 
      cell nuclei of all studied primate species, despite evolutionary chromosome 
      reshuffling. In contrast, in primate fibroblast nuclei evolutionary 
      translocations, fissions and fusions resulted in positional shifts of orthologous 
      chromosome segments, thus arguing against a functional role of chromosome 
      size-dependent spatial chromatin arrangements and for geometrical constraints in 
      flat-ellipsoidal fibroblast nuclei. Notably, in both cell types, regions of 
      rearranged chromosomes with distinct differences in gene density showed polarized 
      arrangements with the more gene-dense segment oriented towards the nuclear 
      interior. Our results indicate that nonrandom breakage and rejoining of 
      preferentially gene-dense chromosomes or chromosome segments may have occurred 
      during evolution.
FAU - Neusser, Michaela
AU  - Neusser M
AD  - Department Biology II, Human Genetics, Ludwig-Maximilians-University, 
      Grosshaderner Strasse 2, 82152 Planegg-Martinsried, Germany.
FAU - Schubel, Verena
AU  - Schubel V
FAU - Koch, Andreas
AU  - Koch A
FAU - Cremer, Thomas
AU  - Cremer T
FAU - Muller, Stefan
AU  - Muller S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070223
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Chromatin)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/metabolism/*ultrastructure
MH  - Chromatin/metabolism/*ultrastructure
MH  - Chromosomes/metabolism/*ultrastructure
MH  - DNA, Ribosomal/metabolism
MH  - *Evolution, Molecular
MH  - Fibroblasts/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Karyotyping
MH  - Lymphocytes/ultrastructure
MH  - Primates/*genetics
MH  - Translocation, Genetic
EDAT- 2007/02/24 09:00
MHDA- 2007/09/29 09:00
CRDT- 2007/02/24 09:00
PHST- 2006/11/02 00:00 [received]
PHST- 2007/01/13 00:00 [accepted]
PHST- 2007/01/03 00:00 [revised]
PHST- 2007/02/24 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2007/02/24 09:00 [entrez]
AID - 10.1007/s00412-007-0099-3 [doi]
PST - ppublish
SO  - Chromosoma. 2007 Jun;116(3):307-20. doi: 10.1007/s00412-007-0099-3. Epub 2007 Feb 
      23.