PMID- 17320888
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20101118
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1147
IP  - 1
DP  - 2007 Apr 13
TI  - In-situ grafting hydrophilic polymer on chitosan modified poly(dimethylsiloxane) 
      microchip for separation of biomolecules.
PG  - 120-6
AB  - In this paper, a simple and green modification method is developed for 
      biomolecules analysis on poly(dimethylsiloxane) (PDMS) microchip with successful 
      depression of nonspecific biomolecules adsorption. 
      O-[(N-succinimdyl)succiny]-o'-methyl-poly(ethylene glycol) was explored to form 
      hydrophilic surface via in-situ grafting onto pre-coated chitosan (Chit) from 
      aqueous solution in the PDMS microchannel. The polysaccharide chains backbone of 
      Chit was strongly attracted onto the surface of PDMS via hydrophobic interaction 
      combined with hydrogen bonding in an alkaline medium. The methyl-poly(ethylene 
      glycol) (mPEG) could produce hydrophilic domains on the mPEG/aqueous interface, 
      which generated brush-like coating in this way and revealed perfect resistance to 
      nonspecific adsorption of biomolecules. This strategy could greatly improve 
      separation efficiency and reproducibility of biomolecules. Amino acids and 
      proteins could be efficiently separated and successfully detected on the coated 
      microchip coupled with end-channel amperometric detection at a copper electrode. 
      In addition, it offered an effective means for preparing biocompatible and 
      hydrophilic surface on microfluidic devices, which may have potential use in the 
      biological analysis.
FAU - Wang, Ai-Jun
AU  - Wang AJ
AD  - Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and 
      Chemical Engineering, Nanjing University, Nanjing 210093, China.
FAU - Xu, Jing-Juan
AU  - Xu JJ
FAU - Chen, Hong-Yuan
AU  - Chen HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070214
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Amino Acids)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Adsorption
MH  - Amino Acids/chemistry/*isolation & purification
MH  - Chitosan/*chemistry
MH  - Coated Materials, Biocompatible/*chemical synthesis/chemistry
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Electrophoresis/methods
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microfluidic Analytical Techniques/*methods
MH  - Polymers/chemistry
MH  - Proteins/chemistry/*isolation & purification
MH  - Reproducibility of Results
MH  - Surface Properties
EDAT- 2007/02/27 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/02/27 09:00
PHST- 2007/01/02 00:00 [received]
PHST- 2007/02/03 00:00 [revised]
PHST- 2007/02/07 00:00 [accepted]
PHST- 2007/02/27 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/02/27 09:00 [entrez]
AID - S0021-9673(07)00276-2 [pii]
AID - 10.1016/j.chroma.2007.02.030 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Apr 13;1147(1):120-6. doi: 10.1016/j.chroma.2007.02.030. 
      Epub 2007 Feb 14.