PMID- 17321328
OWN - NLM
STAT- MEDLINE
DCOM- 20070402
LR  - 20070226
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 173
IP  - 2
DP  - 2007 Mar
TI  - Utility of a multiprobe fluorescence in situ hybridization assay in the detection 
      of superficial urothelial bladder cancer.
PG  - 131-5
AB  - We evaluated the performance of a multiprobe FISH (fluorescence in situ 
      hybridization) assay for noninvasive detection of superficial urothelial 
      carcinoma (UC) in the bladder, in comparison to urinary cytology. Voided urine 
      samples from 74 patients with superficial UC were analyzed by both techniques. 
      Urine samples from 19 patients with muscle-invasive tumors and from 19 healthy 
      control subjects were also studied. For FISH analysis, labeled probes for 
      chromosomes 3, 7, 9, and 17 were used to assess chromosomal abnormalities 
      indicative of malignancy. We found a significant difference between the overall 
      sensitivity of FISH and cytology in superficial UC detection (70.3 versus 35.1%, 
      respectively; P < 0.0001). This significant difference was maintained when 
      superficial UCs were broken down into low grade (52.8 versus 13.9%, respectively; 
      P < 0.0005) and high grade (86.8 versus 55.3%, respectively; P < 0.0015) tumors. 
      Overall specificity was 100% for cytology and 94.7% for FISH (difference not 
      significant). Of patients with suspicious cytology, 69% were positive by FISH. 
      Together, these findings suggest that FISH assay for chromosomes 3, 7, 9, and 17 
      has a higher sensitivity than cytology and a similar specificity in the detection 
      of superficial UC--which could be useful for reducing some cystoscopies in the 
      accurate follow-up usually performed in these patients.
FAU - Marin-Aguilera, Mercedes
AU  - Marin-Aguilera M
AD  - Department of Urology, Hospital Clinic, Institut d'Investigacions Biomediques 
      August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Villarroel 170, 08036 
      Barcelona, Spain.
FAU - Mengual, Lourdes
AU  - Mengual L
FAU - Ribal, Maria Jose
AU  - Ribal MJ
FAU - Burset, Moises
AU  - Burset M
FAU - Arce, Yolanda
AU  - Arce Y
FAU - Ars, Elisabet
AU  - Ars E
FAU - Oliver, Artur
AU  - Oliver A
FAU - Villavicencio, Humberto
AU  - Villavicencio H
FAU - Algaba, Ferran
AU  - Algaba F
FAU - Alcaraz, Antonio
AU  - Alcaraz A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Cytodiagnosis/methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics/urine
EDAT- 2007/02/27 09:00
MHDA- 2007/04/03 09:00
CRDT- 2007/02/27 09:00
PHST- 2006/07/31 00:00 [received]
PHST- 2006/10/02 00:00 [revised]
PHST- 2006/10/13 00:00 [accepted]
PHST- 2007/02/27 09:00 [pubmed]
PHST- 2007/04/03 09:00 [medline]
PHST- 2007/02/27 09:00 [entrez]
AID - S0165-4608(06)00710-2 [pii]
AID - 10.1016/j.cancergencyto.2006.10.011 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Mar;173(2):131-5. doi: 
      10.1016/j.cancergencyto.2006.10.011.