PMID- 17321702
OWN - NLM
STAT- MEDLINE
DCOM- 20070807
LR  - 20171116
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 122
IP  - 1-2
DP  - 2007 May 16
TI  - The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine 
      reproductive and respiratory syndrome virus co-inoculation-associated lymphocyte 
      apoptosis in vitro.
PG  - 72-82
AB  - Lymphoid depletion of various lymphoid organs is one of the major lesions in pigs 
      suffering from postweaning multisystemic wasting syndrome (PMWS). The 
      co-existence of porcine circovirus type 2 (PCV2) and porcine reproductive and 
      respiratory syndrome virus (PRRSV) in PMWS-affected pigs along with the more 
      severe and wider range of lymphocyte depletion of lymphoid organs in PCV2 and 
      PRRSV dually-inoculated pigs imply that PCV2 and PRRSV may interact in the 
      pathogenesis of PMWS. The mechanism for the development of lymphoid depletion in 
      PMWS-affected pigs remains controversial. The objective of the present study was 
      to evaluate and compare the effects of inoculation of both viruses, singularly or 
      in combination, on swine splenic macrophages (SMs) and co-cultured splenic (SLs) 
      and peripheral blood (PBLs) lymphocytes in vitro. A significant reduction in the 
      survival rate and increase in the apoptotic rate of the co-cultured SLs and PBLs 
      and concurrent increase in the expression levels of Fas ligand (FasL) in SMs and 
      Fas in co-cultured SLs and PBLs were demonstrated in PRRSV alone- and PCV2 and 
      PRRSV dually-inoculated groups with the latter more prominent. The increased FasL 
      was proven capable of inducing Fas/FasL-mediated apoptosis in co-cultured 
      FasL-sensitive Jurkat T cells. The de novo expression and production of FasL in 
      PCV2 and PRRSV dually-inoculated SMs and concurrently increased surface 
      expression of Fas in co-cultured lymphocytes may contribute, at least partially, 
      to lymphoid depletion in PMWS-affected pigs with PCV2 and PRRSV dual infection.
FAU - Chang, Hui-Wen
AU  - Chang HW
AD  - Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 
      106, Taiwan, ROC.
FAU - Jeng, Chian-Ren
AU  - Jeng CR
FAU - Lin, Chun-Ming
AU  - Lin CM
FAU - Liu, Jiuan Judy
AU  - Liu JJ
FAU - Chang, Chih-Cheng
AU  - Chang CC
FAU - Tsai, Yi-Chieh
AU  - Tsai YC
FAU - Chia, Mi-Yuan
AU  - Chia MY
FAU - Pang, Victor Fei
AU  - Pang VF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070123
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Fas Ligand Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Circovirus/*metabolism
MH  - Fas Ligand Protein/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Jurkat Cells
MH  - Lymphocytes/*metabolism/*virology
MH  - Macrophages/metabolism/virology
MH  - Male
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Spleen/cytology
MH  - Swine
MH  - fas Receptor/genetics/*metabolism
EDAT- 2007/02/27 09:00
MHDA- 2007/08/08 09:00
CRDT- 2007/02/27 09:00
PHST- 2006/10/17 00:00 [received]
PHST- 2007/01/14 00:00 [revised]
PHST- 2007/01/16 00:00 [accepted]
PHST- 2007/02/27 09:00 [pubmed]
PHST- 2007/08/08 09:00 [medline]
PHST- 2007/02/27 09:00 [entrez]
AID - S0378-1135(07)00035-1 [pii]
AID - 10.1016/j.vetmic.2007.01.013 [doi]
PST - ppublish
SO  - Vet Microbiol. 2007 May 16;122(1-2):72-82. doi: 10.1016/j.vetmic.2007.01.013. 
      Epub 2007 Jan 23.