PMID- 17323198
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20081121
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 152
IP  - 5
DP  - 2007
TI  - Diversity and evolution of a newly emerged North American Type 1 porcine 
      arterivirus: analysis of isolates collected between 1999 and 2004.
PG  - 1009-17
AB  - European-like Type 1 porcine reproductive and respiratory syndrome virus (PRRSV) 
      isolates, known as North American (NA) Type 1 PRRSV, appeared in United States 
      (U.S.) swine herds in 1999. Their diversity and evolution were studied over a 
      five-year period by constructing phylogenetic trees using nsp2 and ORF5 sequences 
      of 20 NA Type 1 isolates, including the only known isolate from Hawaii. All but 
      two of the isolates possessed the same 51-nt deletion in nsp2, suggesting a 
      clonal origin. Parsimony and distance analysis showed that viruses could be 
      placed into two distinct sub-clades, which were similar for both nsp2 and ORF5. 
      An incongruity between the two trees identified one isolate, 04-41, as the 
      product of recombination. Recombination analysis using SimPlot identified a break 
      point located downstream of the nsp2/3 junction. Results from this study suggest 
      that NA Type 1 PRRSV in the U.S. is a well-established and rapidly evolving 
      group. However, the forces driving genetic diversity and separation are complex 
      and remain to be elucidated.
FAU - Fang, Y
AU  - Fang Y
AD  - Center for Infectious Disease Research and Vaccinology, Department of Veterinary 
      Science, South Dakota State University, Brookings, SD, USA.
FAU - Schneider, P
AU  - Schneider P
FAU - Zhang, W P
AU  - Zhang WP
FAU - Faaberg, K S
AU  - Faaberg KS
FAU - Nelson, E A
AU  - Nelson EA
FAU - Rowland, R R R
AU  - Rowland RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070226
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Evolution, Molecular
MH  - *Genes, Viral
MH  - Genetic Variation
MH  - Open Reading Frames/genetics
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*genetics
MH  - Recombination, Genetic
MH  - Swine
MH  - United States
MH  - Viral Nonstructural Proteins/genetics
EDAT- 2007/02/27 09:00
MHDA- 2007/09/15 09:00
CRDT- 2007/02/27 09:00
PHST- 2006/09/27 00:00 [received]
PHST- 2007/01/03 00:00 [accepted]
PHST- 2007/02/27 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2007/02/27 09:00 [entrez]
AID - 10.1007/s00705-007-0936-y [doi]
PST - ppublish
SO  - Arch Virol. 2007;152(5):1009-17. doi: 10.1007/s00705-007-0936-y. Epub 2007 Feb 
      26.