PMID- 17324335
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20070227
IS  - 1557-9190 (Print)
IS  - 1557-9190 (Linking)
VI  - 7
IP  - 4
DP  - 2007 Jan
TI  - Sequential analysis of chromosome aberrations in multiple myeloma during disease 
      progression.
PG  - 280-5
AB  - PURPOSE: Several chromosomal aberrations have been associated with molecular 
      pathogenesis and classification of multiple myeloma. It is not known whether the 
      expression of abnormal karyotypes is consistent in patients during disease 
      progression. Herein, we report on sequential analysis of conventional 
      cytogenetics as well as fluorescence in situ hybridization (FISH) data of 79 bone 
      marrow specimens from 38 patients with myeloma who were longitudinally followed. 
      PATIENTS AND METHODS: We determined and characterized the development of 
      additional chromosomal aberrations during progressive disease. RESULTS: Overall, 
      conventional cytogenetics detected an abnormal karyotype in 42% of the samples, 
      whereas this increased to 69% by FISH. Among the cases with an abnormal 
      conventional karyotype, 52% had a hyperdiploid subtype of myeloma. Progressive 
      disease was correlated with an increased complexity of genetic abnormalities, 
      which in the majority, consisted of structural aberrations acquired in later 
      stages of disease. Using conventional cytogenetics, rearrangements of chromosome 
      1 were the most common structural abnormality (15%). In the majority, these 
      rearrangements consisted of unbalanced translocations of 1p and 1q; however, no 
      specific locus was predominantly affected. Second in frequency were structural 
      aberrations of chromosomes 8 and 17 (6%). The frequency of del(13q) by FISH was 
      40% and did not increase in later stages of the disease, suggesting that del(13q) 
      is not a genetic event associated with disease progression. Change of ploidy 
      category during disease progression occurred in a minority of the cases. 
      CONCLUSION: This study supports the notion that cytogenetic abnormalities in 
      multiple myeloma are not random. Particular chromosomal alterations are 
      associated with disease progression, whereas others show a stable pattern during 
      the course of the disease.
FAU - Wu, Ka Lung
AU  - Wu KL
AD  - Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - Beverloo, Berna
AU  - Beverloo B
FAU - Velthuizen, Sandra J C M
AU  - Velthuizen SJ
FAU - Sonneveld, Pieter
AU  - Sonneveld P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Lymphoma Myeloma
JT  - Clinical lymphoma & myeloma
JID - 101256500
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Longitudinal Studies
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*diagnosis/*genetics/pathology
MH  - Ploidies
MH  - Sensitivity and Specificity
EDAT- 2007/02/28 09:00
MHDA- 2007/04/04 09:00
CRDT- 2007/02/28 09:00
PHST- 2007/02/28 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2007/02/28 09:00 [entrez]
AID - S1557-9190(11)70061-0 [pii]
AID - 10.3816/CLM.2007.n.003 [doi]
PST - ppublish
SO  - Clin Lymphoma Myeloma. 2007 Jan;7(4):280-5. doi: 10.3816/CLM.2007.n.003.