PMID- 17325486
OWN - NLM
STAT- MEDLINE
DCOM- 20070426
LR  - 20151119
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 31
IP  - 3
DP  - 2007 Mar
TI  - Splenic marginal-zone lymphoma: one or more entities? A histologic, 
      immunohistochemical, and molecular study of 42 cases.
PG  - 438-46
AB  - We analyzed 42 splenic marginal-zone lymphoma (SMZL) cases diagnosed on 
      splenectomy specimens after established World Health Organization criteria. A 
      predominantly nodular growth pattern was observed in 24 cases; the remainder 
      showed predominantly (11/42) or exclusively (7/42) diffuse infiltration. 
      Twenty-one cases showed the "classic" biphasic appearance; 13 cases exhibited 
      marginal-zone morphology; finally, 8 cases were composed predominantly of small 
      cells. CD21 and CD35 were expressed by 12/42 and 17/38 cases, respectively. 
      DBA.44 was detected in 24/42 cases. Seventeen of 37 cases were surface IgD 
      (SIgD)-positive. Twenty-one of 22 analyzed cases were SIgM-positive (12/21 
      coexpressed SIgD). Five of 37 cases were SIgG-positive. CD27 staining was 
      observed in 21/35 cases; 7/18 CD27-positive cases coexpressed SIgD; 7/14 
      CD27-negative cases were SIgD-positive. Forty IGHV-D-J rearrangements were 
      amplified in 34/42 cases: the IGHV4-34 gene predominated, followed by IGHV1-2. 
      Using the 98% homology cut-off, 25/40 (62.5%) IGHV sequences were considered as 
      "mutated": 10/11 cases with monomorphous, marginal-zone morphology were 
      IGHV-mutated; in contrast, 4/6 cases with monomorphous, small-cell morphology 
      were IGHV-unmutated. Five of 7 cases expressing IGHV1 subgroup genes had biphasic 
      morphology, whereas 6/9 IGHV3-expressing cases had monomorphous, marginal-zone 
      morphology. Most IGHV-mutated cases (14/20; 70%) were SIgD-negative; in contrast, 
      8/11 IGHV-unmutated cases expressed SIgD. CD27 was detected in 10/17 IGHV-mutated 
      and 6/10 IGHV-unmutated cases. Seven of 11 CD27-negative cases were IGHV-mutated; 
      5/7 CD27-negative/IGHV-mutated cases expressed DBA.44. These results confirm the 
      considerable histologic, immunohistochemical, and molecular heterogeneity of SMZL 
      and indicate an origin from the diverse resident B-cell populations of the normal 
      SMZ.
FAU - Papadaki, Theodora
AU  - Papadaki T
AD  - Hemopathology Department, Evangelismos Hospital, Athens, Greece.
FAU - Stamatopoulos, Kostas
AU  - Stamatopoulos K
FAU - Belessi, Chrysoula
AU  - Belessi C
FAU - Pouliou, Evi
AU  - Pouliou E
FAU - Parasi, Aikaterini
AU  - Parasi A
FAU - Douka, Vassiliki
AU  - Douka V
FAU - Laoutaris, Nikolaos
AU  - Laoutaris N
FAU - Fassas, Athanasios
AU  - Fassas A
FAU - Anagnostopoulos, Achilles
AU  - Anagnostopoulos A
FAU - Anagnostou, Dimitra
AU  - Anagnostou D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm/analysis
MH  - Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics
MH  - Gene Rearrangement, B-Lymphocyte, Light Chain/genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Light Chains/genetics
MH  - Immunophenotyping
MH  - Lymphoma/genetics/metabolism/*pathology
MH  - RNA, Neoplasm/analysis
MH  - Spleen/*pathology
MH  - Splenectomy
MH  - Splenic Neoplasms/genetics/metabolism/*pathology
EDAT- 2007/02/28 09:00
MHDA- 2007/04/27 09:00
CRDT- 2007/02/28 09:00
PHST- 2007/02/28 09:00 [pubmed]
PHST- 2007/04/27 09:00 [medline]
PHST- 2007/02/28 09:00 [entrez]
AID - 00000478-200703000-00013 [pii]
AID - 10.1097/01.pas.0000213419.08009.b0 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2007 Mar;31(3):438-46. doi: 10.1097/01.pas.0000213419.08009.b0.