PMID- 17326775
OWN - NLM
STAT- MEDLINE
DCOM- 20070427
LR  - 20220316
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 20
IP  - 4
DP  - 2007 Apr
TI  - Determination of an adequate perfusion pressure for continuous dual vessel 
      hypothermic machine perfusion of the rat liver.
PG  - 343-52
AB  - Hypothermic machine perfusion (HMP) provides better protection against ischemic 
      damage of the kidney compared to cold-storage. The required perfusion pressures 
      needed for optimal HMP of the liver are, however, unknown. Rat livers were 
      preserved in University of Wisconsin organ preservation solution enriched with 
      acridine orange (AO) to stain viable cells and propidium iodide (PI) to detect 
      dead cells. Perfusion pressures of 12.5%, 25% or 50% of physiologic perfusion 
      pressures were compared. Intravital fluorescence microscopy was used to assess 
      liver perfusion by measuring the percentage of AO staining. After 1-h, the 
      perfusion pressure of 12.5% revealed 72% +/- 3% perfusion of mainly the acinary 
      zones one and two. The perfusion pressure of 25% and 50% showed complete 
      perfusion. Furthermore, 12.5% showed 14.7 +/- 3.6, 25% showed 3.7 +/- 0.9, and 
      50% showed 11.2 +/- 1.4 PI positive cells. One hour was followed by another 
      series of experiments comprising 24-h preservation. In comparison with 24-h 
      cold-storage, HMP at 25% showed less PI positive cells and HMP at 50% showed more 
      PI positive cells. In summary, perfusion at 25% showed complete perfusion, 
      demonstrated by AO staining, with minimal cellular injury, shown with PI. This 
      study indicates that fine-tuning of the perfusion pressure is crucial to balance 
      (in)complete perfusion and endothelial injury.
FAU - 't Hart, Nils A
AU  - 't Hart NA
AD  - Surgery Research Laboratory, University Medical Center Groningen, University of 
      Groningen, Groningen, The Netherlands. n.a.hart@path.umcg.nl
FAU - der van Plaats, Arjan
AU  - der van Plaats A
FAU - Leuvenink, Henri G D
AU  - Leuvenink HG
FAU - van Goor, Harry
AU  - van Goor H
FAU - Wiersema-Buist, Janneke
AU  - Wiersema-Buist J
FAU - Verkerke, Gijsbertus J
AU  - Verkerke GJ
FAU - Rakhorst, Gerhard
AU  - Rakhorst G
FAU - Ploeg, Rutger J
AU  - Ploeg RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 36015-30-2 (Propidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cell Survival
MH  - *Hypothermia, Induced
MH  - Liver/*physiopathology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Organ Preservation/*methods
MH  - Perfusion/*methods
MH  - Pressure
MH  - Propidium
MH  - Rats
MH  - Staining and Labeling
EDAT- 2007/03/01 09:00
MHDA- 2007/04/28 09:00
CRDT- 2007/03/01 09:00
PHST- 2007/03/01 09:00 [pubmed]
PHST- 2007/04/28 09:00 [medline]
PHST- 2007/03/01 09:00 [entrez]
AID - TRI433 [pii]
AID - 10.1111/j.1432-2277.2006.00433.x [doi]
PST - ppublish
SO  - Transpl Int. 2007 Apr;20(4):343-52. doi: 10.1111/j.1432-2277.2006.00433.x.