PMID- 17328142 OWN - NLM STAT- MEDLINE DCOM- 20080902 LR - 20161018 IS - 0250-3301 (Print) IS - 0250-3301 (Linking) VI - 27 IP - 11 DP - 2006 Nov TI - [Spatial distribution of nitrifying bacteria communities in suspended carrier biofilm]. PG - 2358-62 AB - Spatial distributions of ammonia oxidizing bacteria (AOB) and nitrobacteria in a renovated suspended carrier biofilm reactor (SCBR) were investigated by using fluorescence in situ hybridization (FISH) with 16S rRNA oligonucleotide probes in combination with confocal laser scanning microscopy (CLSM). Three bench-scale structurally identical SCBR reactors were operated under different ratios of COD to NH4(+) -N in influents, 5, 10 and 15, respectively. Each SCBR reactor was consisted of a 6 L of aeration basin and a 2L of clarifier, with the hydraulic retention time (HRT) of 1.0h. The monitoring results showed that the thickness of biofilm in the SCBR was about 80 to 120 micron. Both the total amount of AOB and nitrobacteria decreased with depth in biofilm, most of the nitrification bacteria communities lied in the upper layer of biofilm, about 20 to 30 micron. The proportion of AOB to all bacteria in biofilm decreased when the ratio of COD to NH4(+) -N increased. FAU - Wang, Rong-Chang AU - Wang RC AD - College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. wangrongchang@mail.tongji.edu.cn FAU - Wen, Xiang-Hua AU - Wen XH FAU - Qian, Yi AU - Qian Y LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Huan Jing Ke Xue JT - Huan jing ke xue= Huanjing kexue JID - 8405344 RN - 0 (RNA, Ribosomal, 16S) RN - 7664-41-7 (Ammonia) SB - IM MH - Ammonia/metabolism MH - Bacteria, Aerobic/classification/genetics/*physiology MH - Biofilms/*growth & development MH - Bioreactors/*microbiology MH - In Situ Hybridization, Fluorescence MH - Microscopy, Confocal MH - Nitrobacter/genetics/metabolism/physiology MH - RNA, Ribosomal, 16S/genetics MH - Waste Disposal, Fluid/*methods EDAT- 2007/03/03 09:00 MHDA- 2008/09/03 09:00 CRDT- 2007/03/03 09:00 PHST- 2007/03/03 09:00 [pubmed] PHST- 2008/09/03 09:00 [medline] PHST- 2007/03/03 09:00 [entrez] PST - ppublish SO - Huan Jing Ke Xue. 2006 Nov;27(11):2358-62.