PMID- 17328901
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20090115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1147
IP  - 1
DP  - 2007 Apr 13
TI  - Novel monolithic poly(phenyl acrylate-co-1,4-phenylene diacrylate) capillary 
      columns for biopolymer chromatography.
PG  - 46-52
AB  - Monolithic capillary columns were prepared by thermally initiated free radical 
      polymerisation of phenyl acrylate (PA) and 1,4-phenylene diacrylate (PDA) in the 
      confines of 200 microm I.D. fused silica capillaries. Polymerisation was 
      performed in the presence of 2-propanol and tetrahydrofuran (THF) as inert 
      diluents (porogens), using alpha,alpha'-azoisobutyronitrile (AIBN) as initiator. 
      Morphology and porosity of the resulting monoliths were comprehensively studied 
      by scanning electron microscopy (SEM), mercury intrusion porosimetry and inverse 
      size-exclusion chromatography (ISEC). The novel poly(phenyl 
      acrylate-co-1,4-phenylene diacrylate) (PA/PDA) monoliths showed high mechanical 
      stability and were successfully applied to the separation of proteins and 
      oligodeoxynucleotides, employing reversed-phase (RP) and ion-pair reversed-phase 
      (IP-RP) conditions, respectively. Maximum loading capacities for cytochrome c and 
      d(pT)(16) were evaluated and found to be in the region of 200 fmol. 
      Batch-to-batch reproducibility was determined for three independently prepared 
      PA/PDA monolithic capillary columns. Relative standard deviations (RSDs) of 
      retention time (t(R)) of 0.7-1.6% for proteins and 0.2-2.5% for d(pT)(12-18) 
      proved high reproducibility of the PA/PDA supports.
FAU - Bisjak, Clemens P
AU  - Bisjak CP
AD  - Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens 
      University, Innrain 52a, 6020 Innsbruck, Austria.
FAU - Lubbad, Said H
AU  - Lubbad SH
FAU - Trojer, Lukas
AU  - Trojer L
FAU - Bonn, Guenther K
AU  - Bonn GK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070214
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acrylates)
RN  - 0 (Biopolymers)
RN  - 0 (Free Radicals)
RN  - 0 (Nucleic Acids)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 0 (poly(phenyl acrylate-co-1,4-phenylene diacrylate))
SB  - IM
MH  - Acrylates/*chemical synthesis/chemistry
MH  - Biopolymers/*isolation & purification
MH  - Chromatography, High Pressure Liquid/*classification
MH  - Free Radicals/chemistry
MH  - Microscopy, Electron, Scanning
MH  - Molecular Structure
MH  - Nucleic Acids/isolation & purification
MH  - Permeability
MH  - Polymers/*chemical synthesis/chemistry
MH  - Porosity
MH  - Proteins/isolation & purification
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet
EDAT- 2007/03/03 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/03/03 09:00
PHST- 2006/11/28 00:00 [received]
PHST- 2007/02/06 00:00 [revised]
PHST- 2007/02/12 00:00 [accepted]
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - S0021-9673(07)00283-X [pii]
AID - 10.1016/j.chroma.2007.02.027 [doi]
PST - ppublish
SO  - J Chromatogr A. 2007 Apr 13;1147(1):46-52. doi: 10.1016/j.chroma.2007.02.027. 
      Epub 2007 Feb 14.