PMID- 17329099 OWN - NLM STAT- MEDLINE DCOM- 20080104 LR - 20070907 IS - 0960-8524 (Print) IS - 0960-8524 (Linking) VI - 98 IP - 18 DP - 2007 Dec TI - Development of cellular absorptive tracers (CATs) for a quantitative characterization of microbial mass in flow systems. PG - 3630-3 AB - A new method was developed for a simple non-destructive characterization of bacterial mass in flow systems. Results of partition and transport experiments showed that adsorption of a CAT molecule into the cellular mass resulted in its retardation during flow, which was a good measure of the biomass quantity and distribution. Three dyes, acridine orange (AO), toluidine blue (TB), and safranin O (SO), were chosen as CATs to demonstrate their utility to quantitatively characterize the biomass, its location and morphology in flow system. The results clearly demonstrated the applicability of AO, TB, and SO as cellular absorptive tracers in columnar flow experiments. FAU - Choi, Jaeyoung AU - Choi J AD - Korea Institute of Science and Technology (KIST)-Gangneung Institute, Gangneung 210-703, South Korea. jchoi@kist.re.kr FAU - Saripalli, Prasad AU - Saripalli P FAU - Meldrum, Deirdre AU - Meldrum D FAU - Lee, Ju Young AU - Lee JY LA - eng PT - Journal Article DEP - 20070227 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 SB - IM MH - Bacteria/*cytology/*metabolism MH - Bacteriological Techniques MH - Biomass MH - *Bioreactors MH - Waste Disposal, Fluid/*methods EDAT- 2007/03/03 09:00 MHDA- 2008/01/05 09:00 CRDT- 2007/03/03 09:00 PHST- 2006/03/16 00:00 [received] PHST- 2006/11/05 00:00 [revised] PHST- 2006/11/08 00:00 [accepted] PHST- 2007/03/03 09:00 [pubmed] PHST- 2008/01/05 09:00 [medline] PHST- 2007/03/03 09:00 [entrez] AID - S0960-8524(06)00625-0 [pii] AID - 10.1016/j.biortech.2006.11.030 [doi] PST - ppublish SO - Bioresour Technol. 2007 Dec;98(18):3630-3. doi: 10.1016/j.biortech.2006.11.030. Epub 2007 Feb 27.