PMID- 17332092
OWN - NLM
STAT- MEDLINE
DCOM- 20070502
LR  - 20110523
IS  - 1087-0571 (Print)
IS  - 1087-0571 (Linking)
VI  - 12
IP  - 3
DP  - 2007 Apr
TI  - Primary cultures of embryonic chicken neurons for sensitive cell-based assay of 
      botulinum neurotoxin: implications for therapeutic discovery.
PG  - 370-7
AB  - Botulinum toxin is an exceedingly potent inhibitor of neurotransmission across 
      the neuromuscular junction, causing flaccid paralysis and death. The potential 
      for misuse of this deadly poison as a bioweapon has added a greater urgency to 
      the search for effective therapeutics. The development of sensitive and efficient 
      cell-based assays for the evaluation of toxin antagonists is crucial to the rapid 
      and successful identification of therapeutic compounds. The authors evaluated the 
      sensitivity of primary cultures from 4 distinct regions of the embryonic chick 
      nervous system to botulinum neurotoxin A (BoNT/A) cleavage of 
      synaptosomal-associated protein of 25 kD (SNAP-25). Although differences in 
      sensitivity were apparent, SNAP-25 cleavage was detectable in neuronal cells from 
      each of the 4 regions within 3 h at BoNT/A concentrations of 1 nM or lower. 
      Co-incubation of chick neurons with BoNT/A and toxin-neutralizing antibodies 
      inhibited SNAP-25 cleavage, demonstrating the utility of these cultures for the 
      assay of BoNT/A antagonists.
FAU - Stahl, Andrea M
AU  - Stahl AM
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 
      21702, USA.
FAU - Ruthel, Gordon
AU  - Ruthel G
FAU - Torres-Melendez, Edna
AU  - Torres-Melendez E
FAU - Kenny, Tara A
AU  - Kenny TA
FAU - Panchal, Rekha G
AU  - Panchal RG
FAU - Bavari, Sina
AU  - Bavari S
LA  - eng
GR  - N01-CO-12400/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070301
PL  - United States
TA  - J Biomol Screen
JT  - Journal of biomolecular screening
JID - 9612112
RN  - 0 (Antibodies)
RN  - 0 (Synaptosomal-Associated Protein 25)
RN  - EC 3.4.24.69 (Botulinum Toxins, Type A)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Biological Assay/*methods
MH  - Botulinum Toxins, Type A/*antagonists & inhibitors/*pharmacology
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - *Drug Evaluation, Preclinical
MH  - Neurons/*cytology/*drug effects
MH  - Sensitivity and Specificity
MH  - Synaptosomal-Associated Protein 25/metabolism
MH  - Time Factors
EDAT- 2007/03/03 09:00
MHDA- 2007/05/03 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/05/03 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - 1087057106299163 [pii]
AID - 10.1177/1087057106299163 [doi]
PST - ppublish
SO  - J Biomol Screen. 2007 Apr;12(3):370-7. doi: 10.1177/1087057106299163. Epub 2007 
      Mar 1.