PMID- 17332148
OWN - NLM
STAT- MEDLINE
DCOM- 20070521
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 53
IP  - 4
DP  - 2007 Apr
TI  - Negative-ion chemical ionization gas chromatography-mass spectrometry assay for 
      enantioselective measurement of amphetamines in oral fluid: application to a 
      controlled study with MDMA and driving under the influence cases.
PG  - 702-10
AB  - BACKGROUND: Enantioselective analysis of amphetamine (AM), methamphetamine (MA), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 
      and 3,4-methylenedioxyethylamphetamine (MDEA) helps interpret toxicological 
      results. Methods have been described for various matrices, but so far not for 
      oral fluid, a matrix of increasing importance in testing for drugs of abuse, 
      especially in the context of driving under the influence of drugs (DUID). 
      METHODS: After dilution with 200 microL carbonate buffer (pH 9), oral fluid 
      samples (10-50 microL) were derivatized with S-heptafluorobutyrylprolyl chloride. 
      The resulting diastereomers were extracted into 100 microL of cyclohexane, 
      separated by gas chromatography (HP-5MS column), and detected by mass 
      spectrometry in the negative-ion chemical ionization mode (GC-NICI-MS). The 
      method was validated and applied to samples from a controlled study with MDMA and 
      from authentic DUID cases. RESULTS: The derivatized AM, MA, MDA, MDMA, and MDEA 
      enantiomers were well separated from each other. The method was linear from 5-250 
      microg/L per enantiomer of MDA and from 25-1250 microg/L per enantiomer of AM, 
      MA, MDMA, and MDEA. With the exception of MDEA, analytical recoveries, 
      repeatability, and intermediate precision were within required limits. The 
      analyte concentrations and enantiomer ratios in the application samples 
      correlated only weakly with corresponding published plasma data. CONCLUSIONS: 
      This sensitive, reliable, and fast GC-NICI-MS assay enantioselectively measures 
      AM, MA, MDA, and MDMA in oral fluid samples. Prediction of plasma concentrations 
      and enantiomer ratios from respective oral fluid data is not possible.
FAU - Peters, Frank T
AU  - Peters FT
AD  - Department of Experimental and Clinical Toxicology, Saarland University, Homburg 
      (Saar), Germany.
FAU - Samyn, Nele
AU  - Samyn N
FAU - Kraemer, Thomas
AU  - Kraemer T
FAU - Riedel, Wim J
AU  - Riedel WJ
FAU - Maurer, Hans H
AU  - Maurer HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070301
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Amphetamines)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Amphetamine-Related Disorders/*diagnosis
MH  - Amphetamines/*analysis/chemistry
MH  - *Automobile Driving
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis/chemistry
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
MH  - Sensitivity and Specificity
MH  - Stereoisomerism
MH  - Substance Abuse Detection/*methods
EDAT- 2007/03/03 09:00
MHDA- 2007/05/22 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/05/22 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - clinchem.2006.081547 [pii]
AID - 10.1373/clinchem.2006.081547 [doi]
PST - ppublish
SO  - Clin Chem. 2007 Apr;53(4):702-10. doi: 10.1373/clinchem.2006.081547. Epub 2007 
      Mar 1.