PMID- 17332290
OWN - NLM
STAT- MEDLINE
DCOM- 20070509
LR  - 20230208
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 13
IP  - 5
DP  - 2007 Mar 1
TI  - Transcriptomic versus chromosomal prognostic markers and clinical outcome in 
      uveal melanoma.
PG  - 1466-71
AB  - PURPOSE: To compare a gene expression-based classifier versus the standard 
      genetic prognostic marker, monosomy 3, for predicting metastasis in uveal 
      melanoma. EXPERIMENTAL DESIGN: Gene expression profiling, fluorescence in situ 
      hybridization (FISH), and array comparative genomic hybridization (aCGH) were 
      done on 67 primary uveal melanomas. Clinical and pathologic prognostic factors 
      were also assessed. Variables were analyzed by Cox proportional hazards, 
      Kaplan-Meier analysis, sensitivity, specificity, positive and negative predictive 
      value, and positive and negative likelihood ratios. RESULTS: The gene 
      expression-based molecular classifier assigned 27 tumors to class 1 (low risk) 
      and 25 tumors to class 2 (high risk). By Cox univariate proportional hazards, 
      class 2 signature (P = 0.0001), advanced patient age (P = 0.01), and scleral 
      invasion (P = 0.007) were the only variables significantly associated with 
      metastasis. Only the class 2 signature was needed to optimize predictive accuracy 
      in a Cox multivariate model. A less significant association with metastasis was 
      observed for monosomy 3 detected by aCGH (P = 0.076) and FISH (P = 0.127). The 
      sensitivity and specificity for the molecular classifier (84.6% and 92.9%, 
      respectively) were superior to monosomy 3 detected by aCGH (58.3% and 85.7%, 
      respectively) and FISH (50.0% and 72.7%, respectively). Positive and negative 
      predictive values (91.7% and 86.7%, respectively) and positive and negative 
      likelihood ratios (11.9 and 0.2, respectively) for the molecular classifier were 
      also superior to those for monosomy 3. CONCLUSIONS: Molecular classification 
      based on gene expression profiling of the primary tumor was superior to monosomy 
      3 and clinicopathologic prognostic factors for predicting metastasis in uveal 
      melanoma.
FAU - Worley, Lori A
AU  - Worley LA
AD  - Department of Ophthalmology and Visual Sciences, Washington University, St. 
      Louis, Missouri 63110, USA.
FAU - Onken, Michael D
AU  - Onken MD
FAU - Person, Erica
AU  - Person E
FAU - Robirds, Diane
AU  - Robirds D
FAU - Branson, Julie
AU  - Branson J
FAU - Char, Devron H
AU  - Char DH
FAU - Perry, Arie
AU  - Perry A
FAU - Harbour, J William
AU  - Harbour JW
LA  - eng
GR  - R01 CA125970/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Female
MH  - *Gene Expression Profiling/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/*pathology
MH  - Middle Aged
MH  - Monosomy
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Transcription, Genetic
MH  - Uveal Neoplasms/*genetics/*pathology
EDAT- 2007/03/03 09:00
MHDA- 2007/05/10 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/05/10 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - 13/5/1466 [pii]
AID - 10.1158/1078-0432.CCR-06-2401 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2007 Mar 1;13(5):1466-71. doi: 10.1158/1078-0432.CCR-06-2401.