PMID- 17332618
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20161124
IS  - 0161-5505 (Print)
IS  - 0161-5505 (Linking)
VI  - 48
IP  - 3
DP  - 2007 Mar
TI  - Intramyocardial Implantation of bone marrow-derived stem cells enhances perfusion 
      in chronic myocardial infarction: dependency on initial perfusion depth and 
      follow-up assessed by gated pinhole SPECT.
PG  - 405-12
AB  - Cell therapy-induced changes in the perfusion of areas of myocardial infarction 
      (MI) remain unclear. This study investigated whether an original pinhole SPECT 
      technique could be applied to a rat MI model to analyze local improvement in 
      myocardial perfusion relating to engraftment sites of bone marrow-derived stem 
      cells (BMSCs). METHODS: Four-month-old MI rats were either untreated (n = 8) or 
      treated (n = 10) by intramyocardial injection of (111)In-labeled BMSCs. Early 
      distribution of (111)In-BMSCs within the MI target was evidenced by dual 
      (111)In/(99m)Tc pinhole SPECT 48 h later. Myocardial perfusion was serially 
      monitored by (99m)Tc-sestamibi pinhole gated SPECT up to 3 mo after 
      transplantation. RESULTS: Forty-eight hours after transplantation, (111)In-BMSCs 
      were observed in all treated rats and in 18 of their 32 underperfused MI segments 
      (<70% sestamibi uptake before transplantation). During the subsequent 3-mo 
      follow-up, the perfusion of MI segments worsened in untreated rats (absolute 
      change in sestamibi uptake, -3% +/- 3%; P < 0.05) but improved in treated rats 
      (+4% +/- 7%; P < 0.05). This perfusion improvement was unrelated to the initial 
      detection of (111)In-BMSCs (+2% +/- 6% in segments with (111)In-BMSCs vs. +5% +/- 
      7% in those without; not statistically significant) but was strongly associated 
      with less severe perfusion defects before transplantation (+6% +/- 6% in segments 
      with 60%-70% sestamibi uptake [n = 19] vs. -1% +/- 6% in those with <60% uptake 
      [n = 13]; P = 0.003). CONCLUSION: When BMSCs are injected within chronic MI, 
      perfusion enhancement predominates in the MI areas showing a high enough residual 
      perfusion before treatment but not in those of the initial cell engraftment, 
      giving evidence of dependency on the perfusion and metabolic environment at 
      implantation sites.
FAU - Tran, Nguyen
AU  - Tran N
AD  - School of Surgery, Faculty of Medicine-UHP, Nancy, France. 
      Nguyen.Tran@medecine.uhp-nancy.fr
FAU - Franken, Philippe R
AU  - Franken PR
FAU - Maskali, Fatiha
AU  - Maskali F
FAU - Nloga, Joseph
AU  - Nloga J
FAU - Maureira, Pablo
AU  - Maureira P
FAU - Poussier, Sylvain
AU  - Poussier S
FAU - Groubatch, Frederique
AU  - Groubatch F
FAU - Vanhove, Chris
AU  - Vanhove C
FAU - Villemot, Jean-Pierre
AU  - Villemot JP
FAU - Marie, Pierre-Yves
AU  - Marie PY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nucl Med
JT  - Journal of nuclear medicine : official publication, Society of Nuclear Medicine
JID - 0217410
RN  - 0 (Indium Radioisotopes)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Chronic Disease
MH  - *Coronary Circulation
MH  - Follow-Up Studies
MH  - Heart/*diagnostic imaging
MH  - Indium Radioisotopes
MH  - Male
MH  - Myocardial Infarction/diagnostic imaging/physiopathology/*surgery
MH  - Rats
MH  - Rats, Wistar
MH  - *Stem Cell Transplantation
MH  - *Tomography, Emission-Computed, Single-Photon
MH  - Ventricular Function, Left
EDAT- 2007/03/03 09:00
MHDA- 2007/04/25 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - 48/3/405 [pii]
PST - ppublish
SO  - J Nucl Med. 2007 Mar;48(3):405-12.