PMID- 17332640
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20070302
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 353
DP  - 2007
TI  - Fluorescence in situ hybridization for the identification of environmental 
      microbes.
PG  - 153-64
AB  - This chapter presents a protocol for the phylogenetic identification of 
      microorganisms in environmental samples (water and sediments) by means of 
      fluorescence in situ hybridization (FISH) with ribosomal RNA-targeted 
      oligonucleotide probes and signal amplification (catalyzed reporter deposition 
      [CARD]). The FISH probes are labeled with the enzyme, horseradish peroxidase 
      (HRP). A subsequent deposition of fluorescently labeled tyramides results in 
      substantially higher signal intensities of target cells than after FISH with 
      probes directly labeled with fluorochromes. Sample preparation and cell 
      permeabilization strategies for various microbial cell wall types are discussed. 
      The custom labeling of tyramides with different fluorochromes is described. A 
      sequential multicolor CARD-FISH protocol is outlined for the simultaneous 
      detection of different phylogenetic groups.
FAU - Pernthaler, Annelie
AU  - Pernthaler A
AD  - Molecular Ecology Department, Max-Planck-Institute for Marine Microbiology, 
      Bremen, Germany.
FAU - Pernthaler, Jakob
AU  - Pernthaler J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Buffers)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Bacteria/genetics/isolation & purification
MH  - Buffers
MH  - *Environmental Microbiology
MH  - Horseradish Peroxidase
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microbiological Techniques/*methods
MH  - Molecular Probe Techniques
MH  - Oligonucleotide Probes/genetics
MH  - RNA, Bacterial/genetics/isolation & purification
EDAT- 2007/03/03 09:00
MHDA- 2007/04/25 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
AID - 1-59745-229-7:153 [pii]
AID - 10.1385/1-59745-229-7:153 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2007;353:153-64. doi: 10.1385/1-59745-229-7:153.