PMID- 17332748
OWN - NLM
STAT- MEDLINE
DCOM- 20070724
LR  - 20220224
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 26
IP  - 6
DP  - 2007 Mar 21
TI  - Structures and physiological roles of 13 integral lipids of bovine heart 
      cytochrome c oxidase.
PG  - 1713-25
AB  - All 13 lipids, including two cardiolipins, one phosphatidylcholine, three 
      phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, 
      were identified in a crystalline bovine heart cytochrome c oxidase (CcO) 
      preparation. The chain lengths and unsaturated bond positions of the fatty acid 
      moieties determined by mass spectrometry suggest that each lipid head group 
      identifies its specific binding site within CcOs. The X-ray structure 
      demonstrates that the flexibility of the fatty acid tails facilitates their 
      effective space-filling functions and that the four phospholipids stabilize the 
      CcO dimer. Binding of dicyclohexylcarbodiimide to the O(2) transfer pathway of 
      CcO causes two palmitate tails of phosphatidylglycerols to block the pathway, 
      suggesting that the palmitates control the O(2) transfer process.The 
      phosphatidylglycerol with vaccenate (cis-Delta(11)-octadecenoate) was found in 
      CcOs of bovine and Paracoccus denitrificans, the ancestor of mitochondrion, 
      indicating that the vaccenate is conserved in bovine CcO in spite of the 
      abundance of oleate (cis-Delta(9)-octadecenoate). The X-ray structure indicates 
      that the protein moiety selects cis-vaccenate near the O(2) transfer pathway 
      against trans-vaccenate. These results suggest that vaccenate plays a critical 
      role in the O(2) transfer mechanism.
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
AD  - Department of Life Science, University of Hyogo, Kamigohri Akoh Hyogo, Japan.
FAU - Aoyama, Hiroshi
AU  - Aoyama H
FAU - Muramoto, Kazumasa
AU  - Muramoto K
FAU - Terada, Hirohito
AU  - Terada H
FAU - Kurauchi, Tsuyoshi
AU  - Kurauchi T
FAU - Tadehara, Yoshiki
AU  - Tadehara Y
FAU - Yamasaki, Akiko
AU  - Yamasaki A
FAU - Sugimura, Takashi
AU  - Sugimura T
FAU - Kurono, Sadamu
AU  - Kurono S
FAU - Tsujimoto, Kazuo
AU  - Tsujimoto K
FAU - Mizushima, Tsunehiro
AU  - Mizushima T
FAU - Yamashita, Eiki
AU  - Yamashita E
FAU - Tsukihara, Tomitake
AU  - Tsukihara T
FAU - Yoshikawa, Shinya
AU  - Yoshikawa S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070301
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Lipids)
RN  - 0 (Oleic Acids)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - GQ72OGU4EV (11-octadecenoic acid)
SB  - IM
MH  - Absorptiometry, Photon
MH  - Animals
MH  - Binding Sites/genetics
MH  - Cattle
MH  - Chromatography, Gas
MH  - Electron Transport Complex IV/*chemistry/metabolism/*physiology
MH  - Evolution, Molecular
MH  - Lipids/*analysis
MH  - *Models, Molecular
MH  - Molecular Structure
MH  - Myocardium/*enzymology
MH  - Oleic Acids/analysis
MH  - Paracoccus denitrificans/chemistry
MH  - Tandem Mass Spectrometry
PMC - PMC1829383
EDAT- 2007/03/03 09:00
MHDA- 2007/07/25 09:00
PMCR- 2008/03/21
CRDT- 2007/03/03 09:00
PHST- 2006/09/16 00:00 [received]
PHST- 2007/01/24 00:00 [accepted]
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/07/25 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
PHST- 2008/03/21 00:00 [pmc-release]
AID - 7601618 [pii]
AID - 10.1038/sj.emboj.7601618 [doi]
PST - ppublish
SO  - EMBO J. 2007 Mar 21;26(6):1713-25. doi: 10.1038/sj.emboj.7601618. Epub 2007 Mar 
      1.