PMID- 17332920
OWN - NLM
STAT- MEDLINE
DCOM- 20070504
LR  - 20220331
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 30
IP  - 4
DP  - 2007 Apr
TI  - Expression of interleukin-11 (IL-11) and IL-11 receptor alpha in human gastric 
      carcinoma and IL-11 upregulates the invasive activity of human gastric carcinoma 
      cells.
PG  - 825-33
AB  - Previous investigations have shown that interleukin-11 (IL-11) and the IL-11 
      receptor (IL-11R) have been correlated with the regulation of tumor progression, 
      cellular growth and differentiation in several malignant tumors. The objectives 
      of this study were to clarify the role of IL-11 and IL-11Ralpha in human gastric 
      carcinoma. IL-11 and IL-11Ralpha were studied in 73 cases of surgically resected 
      human gastric adenocarcinomas by immunohistochemistry. The invasive activity and 
      cell signaling pathway of gastric carcinoma cell lines were also examined. Among 
      the 73 cases of adenocarcinoma, 53 (72.6%) and 47 cases (64.4%) showed positive 
      staining in carcinoma cells for the IL-11 and IL-11Ralpha proteins, respectively. 
      Histologically, IL-11 expression correlated only with Lauren's classification 
      (p<0.05). The expression of IL-11Ralpha correlated with the grade of tumor 
      invasion (p<0.05) and vessel infiltration (p<0.01). All of the four gastric 
      carcinoma cell lines expressed both IL-11 and IL-11Ralpha proteins in western 
      blot analysis. Recombinant human IL-11 (rhIL-11) promoted the migration of SCH 
      cells by the activation of the phosphatidylinositol-3 kinase pathway. Wortmannin 
      diminished the promotion of chemotactic motility and invasive activity by 
      rhIL-11. These findings suggest that the IL-11/IL-11R pathway plays an important 
      role in the progression and the differentiation of human gastric carcinomas.
FAU - Nakayama, Toshiyuki
AU  - Nakayama T
AD  - Department of Tumor and Diagnostic Pathology, Nagasaki University Graduate School 
      of Biomedical Sciences, Nagasaki 852-8523, Japan. toshi-n@nagasaki-u.ac.jp
FAU - Yoshizaki, Ayumi
AU  - Yoshizaki A
FAU - Izumida, Seiya
AU  - Izumida S
FAU - Suehiro, Tomoyuki
AU  - Suehiro T
FAU - Miura, Shiro
AU  - Miura S
FAU - Uemura, Takashi
AU  - Uemura T
FAU - Yakata, Yuichi
AU  - Yakata Y
FAU - Shichijo, Kazuko
AU  - Shichijo K
FAU - Yamashita, Shunichi
AU  - Yamashita S
FAU - Sekin, Ichiro
AU  - Sekin I
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Androstadienes)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Interleukin-11)
RN  - 0 (Interleukin-11 Receptor alpha Subunit)
RN  - 0 (Recombinant Proteins)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Adenocarcinoma/chemistry/metabolism/*pathology
MH  - Androstadienes/pharmacology
MH  - Chemotaxis/drug effects
MH  - Cytokine Receptor gp130/analysis/metabolism
MH  - Humans
MH  - Interleukin-11/analysis/*metabolism
MH  - Interleukin-11 Receptor alpha Subunit/analysis/*metabolism
MH  - Neoplasm Invasiveness
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Recombinant Proteins/pharmacology
MH  - Stomach Neoplasms/chemistry/metabolism/*pathology
MH  - Up-Regulation
MH  - Wortmannin
EDAT- 2007/03/03 09:00
MHDA- 2007/05/05 09:00
CRDT- 2007/03/03 09:00
PHST- 2007/03/03 09:00 [pubmed]
PHST- 2007/05/05 09:00 [medline]
PHST- 2007/03/03 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2007 Apr;30(4):825-33.