PMID- 17335079
OWN - NLM
STAT- MEDLINE
DCOM- 20070718
LR  - 20181201
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 85
IP  - 5
DP  - 2007 Apr
TI  - Embryonic stem cells produce neurotrophins in response to cerebral tissue 
      extract: Cell line-dependent differences.
PG  - 1057-64
AB  - In the present study, we compare the capacity of two different embryonic stem 
      (ES) cell lines to secrete neurotrophins in response to cerebral tissue extract 
      derived from healthy or injured rat brains. The intrinsic capacity of the 
      embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release 
      brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the 
      release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 
      and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. 
      Conditioning of cell lines with cerebral tissue extract derived from healthy or 
      fluid percussion-injured rat brains resulted in a significant time-dependent 
      increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 
      cells was more pronounced when cells were incubated with brain extract derived 
      from injured brain. However, differences in neurotrophin release associated with 
      the origin of brain extract were at no time statistically significant. 
      Neutrophin-3 and NGF release was inhibited when cell lines were exposed to 
      cerebral tissue extract. The magnitude of the response to cerebral tissue extract 
      was dependent on the intrinsic capacity of the cell lines to release 
      neurotrophins. Our results clearly demonstrate significant variations in the 
      intrinsic capability of different stem cell lines to produce neurotrophic 
      factors. Furthermore, a significant modulation of neurotrophic factor release was 
      observed following conditioning of cell lines with tissue extract derived from 
      rat brains. A significant modulation of neurotrophin release dependent on the 
      source of cerebral tissue extract used was not observed.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Bentz, Kristine
AU  - Bentz K
AD  - Institute of Developmental Genetics, GSF-National Research Centre for Environment 
      and Health, Munich, Germany.
FAU - Molcanyi, Marek
AU  - Molcanyi M
FAU - Riess, Peter
AU  - Riess P
FAU - Elbers, Andrea
AU  - Elbers A
FAU - Pohl, Esther
AU  - Pohl E
FAU - Sachinidis, Agapios
AU  - Sachinidis A
FAU - Hescheler, Jurgen
AU  - Hescheler J
FAU - Neugebauer, Edmund
AU  - Neugebauer E
FAU - Schafer, Ute
AU  - Schafer U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cell Extracts)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Brain Chemistry
MH  - Brain Tissue Transplantation/methods
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Extracts/pharmacology
MH  - Cell Line
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Culture Media, Conditioned/pharmacology
MH  - Embryonic Stem Cells/*metabolism
MH  - Mice
MH  - Nerve Growth Factors/*metabolism
MH  - Nerve Regeneration/drug effects/physiology
MH  - Neurotrophin 3/metabolism
MH  - Stem Cell Transplantation/methods
MH  - Time Factors
EDAT- 2007/03/06 09:00
MHDA- 2007/07/19 09:00
CRDT- 2007/03/06 09:00
PHST- 2007/03/06 09:00 [pubmed]
PHST- 2007/07/19 09:00 [medline]
PHST- 2007/03/06 09:00 [entrez]
AID - 10.1002/jnr.21219 [doi]
PST - ppublish
SO  - J Neurosci Res. 2007 Apr;85(5):1057-64. doi: 10.1002/jnr.21219.