PMID- 17335378
OWN - NLM
STAT- MEDLINE
DCOM- 20070430
LR  - 20151119
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 78
IP  - 3
DP  - 2007 Mar
TI  - Nicotine and lipopolysaccharide affect cytokine expression from gingival 
      fibroblasts.
PG  - 533-41
AB  - BACKGROUND: This in vitro study investigated the influence of nicotine, 
      lipopolysaccharide (LPS), and a combination of both agents on cytokine expression 
      from human gingival fibroblasts (HGFs). METHODS: HGFs were exposed for 48 hours 
      to 250 microg/ml nicotine, 1 microg/ml Porphyromonas gingivalis LPS, or both. The 
      expression of multiple cytokines was detected in the HGFs conditioned media using 
      cytokine protein arrays. RESULTS: The untreated HGFs expressed several cytokines, 
      which included relatively high levels of interleukin (IL)-6, IL-8, and monocyte 
      chemoattractant protein-1 (MCP-1). They also expressed low levels of 
      growth-regulated oncogene (GRO), IL-3, and IL-10. Nicotine had the greatest 
      effect on the expression of GRO-alpha, IL-7, IL-10, and IL-15 compared to the 
      untreated control. P. gingivalis LPS had the greatest effect on the expression of 
      GRO-alpha; IL-7; IL-10; and RANTES (regulated on activation, normal T-cell 
      expressed, and presumably secreted) compared to the untreated control. The 
      combination of both agents had the biggest impact on the expression of GRO-alpha, 
      IL-7, IL-10, IL-15, RANTES, and interferon-gamma (IFN-gamma) compared to the 
      untreated control. CONCLUSION: HGFs exposed to nicotine, P. gingivalis LPS, or a 
      combination of both agents increased the expression of multiple cytokines.
FAU - Almasri, Amjad
AU  - Almasri A
AD  - Department of Periodontics, Indiana University School of Dentistry, Indianapolis, 
      IN, USA.
FAU - Wisithphrom, Kessiri
AU  - Wisithphrom K
FAU - Windsor, L Jack
AU  - Windsor LJ
FAU - Olson, Byron
AU  - Olson B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nicotinic Agonists)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CCL5/biosynthesis
MH  - Chemokine CXCL1
MH  - Chemokines, CXC/biosynthesis
MH  - Cytokines/*biosynthesis
MH  - Fibroblasts/drug effects/metabolism
MH  - Gingiva/cytology/*drug effects/metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukins/biosynthesis
MH  - Lipopolysaccharides/toxicity
MH  - Nicotine/*toxicity
MH  - Nicotinic Agonists/*toxicity
MH  - Porphyromonas gingivalis
MH  - Protein Array Analysis
EDAT- 2007/03/06 09:00
MHDA- 2007/05/01 09:00
CRDT- 2007/03/06 09:00
PHST- 2007/03/06 09:00 [pubmed]
PHST- 2007/05/01 09:00 [medline]
PHST- 2007/03/06 09:00 [entrez]
AID - 10.1902/jop.2007.060296 [doi]
PST - ppublish
SO  - J Periodontol. 2007 Mar;78(3):533-41. doi: 10.1902/jop.2007.060296.