PMID- 17335401
OWN - NLM
STAT- MEDLINE
DCOM- 20070612
LR  - 20191210
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 13
IP  - 4
DP  - 2007 Apr
TI  - Spontaneous large volume adipose tissue generation from a vascularized pedicled 
      fat flap inside a chamber space.
PG  - 673-81
AB  - A novel method of spontaneous generation of new adipose tissue from an existing 
      fat flap is described. A defined volume of fat flap based on the superficial 
      inferior epigastric vascular pedicle in the rat was elevated and inset into a 
      hollow plastic chamber implanted subcutaneously in the groin of the rat. The 
      chamber walls were either perforated or solid and the chambers either contained 
      poly(D,L-lactic-co-glycolic acid) (PLGA) sponge matrix or not. The contents were 
      analyzed after being in situ for 6 weeks. The total volume of the flap tissue in 
      all groups except the control groups, where the flap was not inserted into the 
      chambers, increased significantly, especially in the perforated chambers (0.08 
      +/- 0.007 mL baseline compared to 1.2 +/- 0.08 mL in the intact ones). Volume 
      analysis of individual component tissues within the flaps revealed that the 
      adipocyte volume increased and was at a maximum in the chambers without PLGA, 
      where it expanded from 0.04 +/- 0.003 mL at insertion to 0.5 +/- 0.08 mL (1250% 
      increase) in the perforated chambers and to 0.16 +/- 0.03 mL (400% increase) in 
      the intact chambers. Addition of PLGA scaffolds resulted in less fat growth. 
      Histomorphometric analysis rather than simple hypertrophy documented an increased 
      number of adipocytes. The new tissue was highly vascularized and no fat necrosis 
      or atypical changes were observed.
FAU - Dolderer, Juergen H
AU  - Dolderer JH
AD  - The Bernard O'Brien Institute of Microsurgery and Department of Plastic and 
      Reconstructive Surgery, St. Vincent's Hospital, Melbourne, Australia.
FAU - Abberton, Keren M
AU  - Abberton KM
FAU - Thompson, Erik W
AU  - Thompson EW
FAU - Slavin, John L
AU  - Slavin JL
FAU - Stevens, Geoffrey W
AU  - Stevens GW
FAU - Penington, Anthony J
AU  - Penington AJ
FAU - Morrison, Wayne A
AU  - Morrison WA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
SB  - IM
MH  - Adipose Tissue/*anatomy & histology/blood supply/*growth & development
MH  - Animals
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Male
MH  - Organ Size
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Surgical Flaps/*pathology/*physiology
MH  - Tissue Culture Techniques/instrumentation/methods
MH  - Tissue Engineering/*instrumentation/*methods
EDAT- 2007/03/06 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/03/06 09:00
PHST- 2007/03/06 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/03/06 09:00 [entrez]
AID - 10.1089/ten.2006.0212 [doi]
PST - ppublish
SO  - Tissue Eng. 2007 Apr;13(4):673-81. doi: 10.1089/ten.2006.0212.