PMID- 1733627
OWN - NLM
STAT- MEDLINE
DCOM- 19920228
LR  - 20190510
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 87
IP  - 1
DP  - 1992 Jan
TI  - Primary structure of a monoclonal kappa chain in myeloma with light chain 
      deposition disease.
PG  - 122-6
AB  - Previous data suggest that structural abnormalities of immunoglobulin light 
      chains may be responsible for non-amyloid light chain deposition disease (LCDD). 
      We report on the complete primary sequence deduced from complementary (c)DNA 
      analysis of a normal-sized kappa chain in a case of myeloma-associated LCDD. The 
      patient's urine contained a kappa type Bence-Jones protein made of monomers and 
      dimers of an unglycosylated kappa chain. The bone marrow myeloma cells contained 
      intracellular kappa and gamma chains by immunofluorescence. Biosynthesis 
      experiments showed the production of normal-sized gamma chains and of kappa 
      chains with the same apparent molecular mass (Mr) in SDS gels as the urinary 
      kappa chain (26,000-27,000). These kappa chains were secreted as assembled IgG 
      molecules and as a large excess of free monomers and dimers. The complete 
      sequence of two identical cDNA clones derived from a normal-sized kappa messenger 
      RNA indicated that this kappa chain belonged to the rare V kappa IV subgroup. The 
      kappa mRNA had an overall normal structure made up of the V kappa IV sequence 
      rearranged to J kappa 1 and followed by a normal constant exon of the Km(3) 
      allotype. The variable region differed from the V kappa IV-J kappa 1 germline 
      sequence by 17 amino acid substitutions. The peculiar sequence of the variable 
      region of this kappa chain of a rare subgroup might relate to its tissue 
      deposition.
FAU - Khamlichi, A A
AU  - Khamlichi AA
AD  - CNRS URA 1172, Laboratory of Molecular Immunology, Faculte des Sciences, 
      University Hospital, Poitiers, France.
FAU - Aucouturier, P
AU  - Aucouturier P
FAU - Silvain, C
AU  - Silvain C
FAU - Bauwens, M
AU  - Bauwens M
FAU - Touchard, G
AU  - Touchard G
FAU - Preud'homme, J L
AU  - Preud'homme JL
FAU - Nau, F
AU  - Nau F
FAU - Cogne, M
AU  - Cogne M
LA  - eng
SI  - GENBANK/M83092
SI  - GENBANK/S47168
SI  - GENBANK/S49998
SI  - GENBANK/S50001
SI  - GENBANK/S72771
SI  - GENBANK/S79311
SI  - GENBANK/S79324
SI  - GENBANK/X64094
SI  - GENBANK/X64095
SI  - GENBANK/X64096
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Immunoglobulin kappa-Chains)
SB  - IM
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Humans
MH  - Hypergammaglobulinemia/*immunology
MH  - Immunoglobulin kappa-Chains/*chemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Multiple Myeloma/*immunology
PMC - PMC1554240
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
PMCR- 1993/01/01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PHST- 1993/01/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.1992.tb06424.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1992 Jan;87(1):122-6. doi: 10.1111/j.1365-2249.1992.tb06424.x.