PMID- 17336291
OWN - NLM
STAT- MEDLINE
DCOM- 20070803
LR  - 20191210
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 563
IP  - 1-3
DP  - 2007 Jun 1
TI  - Pharmacological characterization of a chronic pruritus model induced by multiple 
      application of 2,4,6-trinitrochlorobenzene in NC mice.
PG  - 233-9
AB  - Female NC/Jic mice were sensitized and challenged repeatedly at 48 h intervals 
      for 10 and 30 days by painting 1% 2,4,6-trinitrochlorobenzene (TNCB) on both 
      ears. Mice challenged with TNCB for 30 days developed an inflammatory dermatitis 
      with high immunoglobulin E (IgE) titer. Histological analysis with acidic 
      Toluidine Blue staining revealed that dermal mast cells markedly differentiated 
      and intensely degranulated, consistent with a dramatic increase in scratching 
      behavior. A significant increase in total scratching events could be observed in 
      mice treated with TNCB for a short period of 10 days. Extending the term of TNCB 
      application to 30 days, the IgE titer and number of mast cells elevated 
      significantly, and thus various drugs were evaluated pharmacologically by using 
      the mice treated with TNCB for 30 days. Terfenadine and cyproheptadine attenuated 
      the chronic scratching behavior. Tacrolimus and dexamethasone were less effective 
      and cromolyn showed no effect. In addition, terfenadine and tacrolimus suppressed 
      the degranulation of mast cells. The present chronic scratching model could be 
      suitable to evaluate drugs effective for suppression of mast cell differentiation 
      and degranulation by irritation, and may represent a promising tool to develop 
      new drugs for inflammatory pruritus associated with, for example, atopic 
      dermatitis.
FAU - Yamashita, Hirotaka
AU  - Yamashita H
AD  - Graduate School of Phrmaceutical Science, Nagoya City University, Tanabe-dori, 
      Mizuho-ku, Nagoya 467-8603, Japan.
FAU - Makino, Toshiaki
AU  - Makino T
FAU - Mizukami, Hajime
AU  - Mizukami H
FAU - Nose, Mitsuhiko
AU  - Nose M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070208
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Antipruritics)
RN  - 2YHB6175DO (Cyproheptadine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7BA5G9Y06Q (Terfenadine)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - Q2WXR1I0PK (Cromolyn Sodium)
RN  - WM0HAQ4WNM (Tacrolimus)
RN  - Z4ZG7O5SZ9 (Picryl Chloride)
SB  - IM
MH  - Animals
MH  - Antipruritics/*pharmacology/therapeutic use
MH  - Cell Degranulation/drug effects
MH  - Chronic Disease
MH  - Cromolyn Sodium/pharmacology
MH  - Cyproheptadine/pharmacology
MH  - Dermatitis, Atopic/chemically induced/*complications/drug 
      therapy/immunology/physiopathology
MH  - Dexamethasone/pharmacology
MH  - *Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Drug Evaluation, Preclinical/*methods
MH  - Female
MH  - Immunoglobulin E/blood
MH  - Mast Cells/drug effects/immunology
MH  - Mice
MH  - *Picryl Chloride
MH  - Pruritus/etiology/immunology/physiopathology/*prevention & control
MH  - Tacrolimus/pharmacology
MH  - Terfenadine/pharmacology
MH  - Time Factors
EDAT- 2007/03/06 09:00
MHDA- 2007/08/04 09:00
CRDT- 2007/03/06 09:00
PHST- 2006/09/13 00:00 [received]
PHST- 2007/01/22 00:00 [revised]
PHST- 2007/01/25 00:00 [accepted]
PHST- 2007/03/06 09:00 [pubmed]
PHST- 2007/08/04 09:00 [medline]
PHST- 2007/03/06 09:00 [entrez]
AID - S0014-2999(07)00142-2 [pii]
AID - 10.1016/j.ejphar.2007.01.078 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2007 Jun 1;563(1-3):233-9. doi: 10.1016/j.ejphar.2007.01.078. 
      Epub 2007 Feb 8.