PMID- 17336942 OWN - NLM STAT- MEDLINE DCOM- 20071026 LR - 20131121 IS - 0006-3223 (Print) IS - 0006-3223 (Linking) VI - 62 IP - 5 DP - 2007 Sep 1 TI - Regulation of serotonin-1A receptor function in inducible brain-derived neurotrophic factor knockout mice after administration of corticosterone. PG - 521-9 AB - BACKGROUND: We examined the effects of a forebrain-specific reduction in brain-derived neurotrophic factor (BDNF) on the regulation of serotonin-1A (5-HT1A) receptor function in serotonergic cell body areas as well as in limbic and cortical structures of mice chronically treated with corticosterone. METHODS: 5-HT1A receptor function, at the level of receptor-G protein interaction, was assessed with quantitative autoradiography of [35S]GTPgammaS binding stimulated by the 5-HT1A receptor agonist 8-OH-DPAT. 5-HT1A receptor number was assessed by measuring the binding of the antagonist radioligand [3H] WAY100635. RESULTS: We observed a significant attenuation of 5-HT1A receptor function, in the absence of a change in receptor number, in the dorsal hippocampus of BDNF knockout versus control mice. There was no difference between control and BDNF knockout mice in 5-HT1A receptor number or function in the dorsal or median raphe nuclei or medial prefrontal cortex or anterior cingulate cortex. Corticosterone treatment of control mice decreased 5-HT1A receptor function in the dorsal and median raphe but not in hippocampus or frontal cortical areas. The regulation of 5HT1A receptor number or function in the dorsal and median raphe by corticosterone was lost in BDNF knockout mice. CONCLUSIONS: Attenuation of BDNF expression in forebrain regions produces differential effects on distinct 5-HT1A receptor populations and on the regulation of these receptor populations by corticosterone. FAU - Hensler, Julie G AU - Hensler JG AD - Department of Pharmacology, University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA. hensler@uthscsa.edu FAU - Advani, Tushar AU - Advani T FAU - Monteggia, Lisa M AU - Monteggia LM LA - eng GR - MH 070727/MH/NIMH NIH HHS/United States GR - MH 52369/MH/NIMH NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20070306 PL - United States TA - Biol Psychiatry JT - Biological psychiatry JID - 0213264 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Piperazines) RN - 0 (Pyridines) RN - 0 (Serotonin Antagonists) RN - 0 (Serotonin Receptor Agonists) RN - 112692-38-3 (Receptor, Serotonin, 5-HT1A) RN - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate)) RN - 71IH826FEG (N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide) RN - 78950-78-4 (8-Hydroxy-2-(di-n-propylamino)tetralin) RN - W980KJ009P (Corticosterone) SB - IM MH - 8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology MH - Animals MH - Anti-Inflammatory Agents/*administration & dosage MH - Autoradiography/methods MH - Brain-Derived Neurotrophic Factor/*deficiency MH - Corticosterone/*administration & dosage MH - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacokinetics MH - Hippocampus/*drug effects MH - Male MH - Mice MH - Mice, Knockout MH - Piperazines/pharmacokinetics MH - Protein Binding/drug effects MH - Pyridines/pharmacokinetics MH - Raphe Nuclei/drug effects/physiology MH - Receptor, Serotonin, 5-HT1A/*physiology MH - Serotonin Antagonists/pharmacokinetics MH - Serotonin Receptor Agonists/pharmacology EDAT- 2007/03/06 09:00 MHDA- 2007/10/30 09:00 CRDT- 2007/03/06 09:00 PHST- 2006/07/18 00:00 [received] PHST- 2006/09/19 00:00 [revised] PHST- 2006/10/12 00:00 [accepted] PHST- 2007/03/06 09:00 [pubmed] PHST- 2007/10/30 09:00 [medline] PHST- 2007/03/06 09:00 [entrez] AID - S0006-3223(06)01319-9 [pii] AID - 10.1016/j.biopsych.2006.10.015 [doi] PST - ppublish SO - Biol Psychiatry. 2007 Sep 1;62(5):521-9. doi: 10.1016/j.biopsych.2006.10.015. Epub 2007 Mar 6.