PMID- 17338270
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20161021
IS  - 1733-1331 (Print)
IS  - 1733-1331 (Linking)
VI  - 55
IP  - 3
DP  - 2006
TI  - Bioactive compounds from Streptomyces nasri and its mutants with special 
      reference to proteopolysaccharides.
PG  - 179-87
AB  - The use of microbial exopolysaccharides (EPS) in the food, pharmaceutical, and 
      chemical industries has steadily increased during the past decade. A bioactive 
      EPS producing microorganism, Streptomyces nasri was isolated from Kuwait tropical 
      soil and the proteopolysaccharide was tested for its antimicrobial activity. The 
      isolate was subjected to ultraviolet (UV) radiation and acridine orange (AO) 
      treatment to select for superior proteopolysaccharide producers. Among eight 
      (five derived from UV exposure and three from AO exposure) morphological variants 
      of Streptomyces nasri, two mutants showed increased EPS production, from 1.8 g/l 
      to 2.3 g/l. The SDS-PAGE profiles of exopolysaccharides were determined. The 
      molecular weight of the proteopolysaccharide ranged from 18 to 200 kDa. Mutants 
      derived from UV exposure produced polysaccharides with higher molecular weight 
      than those derived from acridine orange exposure. Acridine orange derived mutants 
      produced lower molecular weight polysaccharides. Culture supernatants have been 
      partially characterized and they show antimicrobial activity against a wide range 
      of microorganisms. The structure of the exopolysaccharide was determined using 
      NMR spectroscopy. The polysaccharide was also tested for cytotoxic activity 
      against human brain tumor cell line using SRB assay.
FAU - Gohar, Yousry
AU  - Gohar Y
AD  - Division of Microbiology, Faculty of Science, Alexandria University, Alexandria, 
      Egypt. ymgoharos@yahoo.com
FAU - Beshay, Usama
AU  - Beshay U
FAU - Daba, Ayman
AU  - Daba A
FAU - Hafez, Elsayed
AU  - Hafez E
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol J Microbiol
JT  - Polish journal of microbiology
JID - 101229003
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Anti-Infective Agents/*chemistry/*isolation & purification/metabolism
MH  - Antibiotics, Antineoplastic/*pharmacology
MH  - Cell Line
MH  - Humans
MH  - Polysaccharides/*chemistry/*isolation & purification/metabolism
MH  - Soil Microbiology
MH  - Streptomyces/chemistry/genetics/*metabolism
MH  - Structure-Activity Relationship
EDAT- 2007/03/07 09:00
MHDA- 2007/03/28 09:00
CRDT- 2007/03/07 09:00
PHST- 2007/03/07 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2007/03/07 09:00 [entrez]
PST - ppublish
SO  - Pol J Microbiol. 2006;55(3):179-87.