PMID- 17339103
OWN - NLM
STAT- MEDLINE
DCOM- 20070710
LR  - 20091016
IS  - 0927-7765 (Print)
IS  - 0927-7765 (Linking)
VI  - 57
IP  - 2
DP  - 2007 Jun 15
TI  - Intracellular distribution of fluorescent probes delivered by vesicles of 
      different lipidic composition.
PG  - 143-51
AB  - In order to study mechanisms involved in liposome-cell interaction, this work 
      attempted to assess the influence of vesicle composition on the delivery of 
      liposomal content to Hela cells. In particular, to evaluate pH-sensitive 
      properties and cell interaction of the prepared liposomes, the lipid formulations 
      contained cholesterol (Chol) and they were varied by using phosphatidylcholines 
      with different purity degree: soy lecithin (SL; 80% phosphatidylcholine), a 
      commercial mixture of soy phosphatidylcholine (P90; 90% phosphatidylcholine) or 
      dipalmitoylphosphatidylcholine (DPPC; 99% of purity). A second series of 
      liposomes also contained stearylamine (SA). Dehydration-rehydration vesicles 
      (DRV) were prepared and then sonicated to decrease vesicle size. Vesicle-cell 
      interactions and liposomal uptake were examined by fluorescence microscopy using 
      carboxyfluorescein (CF) and phosphatidylethanolamine-dioleoyl-sulforhodamine B 
      (Rho-PE) as fluorescent markers. Fluorescence dequenching assay was used to study 
      the influence of pH on CF release from the liposomal formulations. Liposome 
      adhesion on the cell surface and internalization were strongly dependent on 
      vesicle bilayer composition. SA vesicles were not endocytosed. DPPC/Chol 
      liposomes were endocytosed but did not release their fluorescent content into the 
      cytosol. SL/Chol and P90/Chol formulations displayed a diffuse cytoplasmic 
      fluorescence of liposomal marker.
FAU - Manconi, Maria
AU  - Manconi M
AD  - Dipartimento Farmaco Chimico Tecnologico, Universita di Cagliari, Via Ospedale 
      72, Cagliari, Italy.
FAU - Isola, Raffaella
AU  - Isola R
FAU - Falchi, Angela Maria
AU  - Falchi AM
FAU - Sinico, Chiara
AU  - Sinico C
FAU - Fadda, Anna Maria
AU  - Fadda AM
LA  - eng
PT  - Journal Article
DEP - 20070206
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Liposomes)
SB  - IM
MH  - Fluorescent Dyes/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - *Lipid Metabolism
MH  - Liposomes
MH  - Microscopy, Fluorescence
EDAT- 2007/03/07 09:00
MHDA- 2007/07/11 09:00
CRDT- 2007/03/07 09:00
PHST- 2006/08/23 00:00 [received]
PHST- 2007/01/12 00:00 [revised]
PHST- 2007/01/24 00:00 [accepted]
PHST- 2007/03/07 09:00 [pubmed]
PHST- 2007/07/11 09:00 [medline]
PHST- 2007/03/07 09:00 [entrez]
AID - S0927-7765(07)00049-5 [pii]
AID - 10.1016/j.colsurfb.2007.01.016 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2007 Jun 15;57(2):143-51. doi: 
      10.1016/j.colsurfb.2007.01.016. Epub 2007 Feb 6.