PMID- 17339438
OWN - NLM
STAT- MEDLINE
DCOM- 20070522
LR  - 20191210
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 6
DP  - 2007 Mar 15
TI  - Migration of CD4 T cells and dendritic cells toward sphingosine 1-phosphate (S1P) 
      is mediated by different receptor subtypes: S1P regulates the functions of murine 
      mature dendritic cells via S1P receptor type 3.
PG  - 3437-46
AB  - Dendritic cells (DCs) and lymphocytes are known to show a migratory response to 
      the phospholipid mediator, sphingosine 1-phosphate (S1P). However, it is unclear 
      whether the same S1P receptor subtype mediates the migration of lymphocytes and 
      DCs toward S1P. In this study, we investigated the involvement of S1P receptor 
      subtypes in S1P-induced migration of CD4 T cells and bone marrow-derived DCs in 
      mice. A potent S1P receptor agonist, the (S)-enantiomer of FTY720-phosphate 
      [(S)-FTY720-P], at 0.1 nM or higher and a selective S1P receptor type 1 (S1P(1)) 
      agonist, SEW2871, at 0.1 muM or higher induced a dose-dependent down-regulation 
      of S1P(1). The pretreatment with these compounds resulted in a significant 
      inhibition of mouse CD4 T cell migration toward S1P. Thus, it is revealed that 
      CD4 T cell migration toward S1P is highly dependent on S1P(1). Mature DCs, when 
      compared with CD4 T cells or immature DCs, expressed a relatively higher level of 
      S1P(3) mRNA. S1P at 10-1000 nM induced a marked migration and significantly 
      enhanced the endocytosis of FITC-dextran in mature but not immature DCs. 
      Pretreatment with (S)-FTY720-P at 0.1 microM or higher resulted in a significant 
      inhibition of S1P-induced migration and endocytosis in mature DCs, whereas 
      SEW2871 up to 100 microM did not show any clear effect. Moreover, we found that 
      S1P-induced migration and endocytosis were at an extremely low level in mature 
      DCs prepared from S1P(3)-knockout mice. These results indicate that S1P regulates 
      migration and endocytosis of murine mature DCs via S1P(3) but not S1P(1).
FAU - Maeda, Yasuhiro
AU  - Maeda Y
AD  - Research Laboratory III (Immunology), Pharmaceuticals Research Division, 
      Mitsubishi Pharma Corporation, 1000 Kamoshida-cho, Aoba-ku, Yokohama, Japan.
FAU - Matsuyuki, Hirofumi
AU  - Matsuyuki H
FAU - Shimano, Kyoko
AU  - Shimano K
FAU - Kataoka, Hirotoshi
AU  - Kataoka H
FAU - Sugahara, Kunio
AU  - Sugahara K
FAU - Chiba, Kenji
AU  - Chiba K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lysophospholipids)
RN  - 0 (Oxadiazoles)
RN  - 0 (Propylene Glycols)
RN  - 0 (Receptors, Lysosphingolipid)
RN  - 0 (S1pr3 protein, mouse)
RN  - 0 (SEW2871)
RN  - 0 (Sphingosine-1-Phosphate Receptors)
RN  - 0 (Thiophenes)
RN  - 26993-30-6 (sphingosine 1-phosphate)
RN  - G926EC510T (Fingolimod Hydrochloride)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology/metabolism
MH  - Cell Movement/drug effects/genetics/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/drug effects/genetics/immunology
MH  - Endocytosis/drug effects/genetics/*immunology
MH  - Fingolimod Hydrochloride
MH  - Immunosuppressive Agents/pharmacology
MH  - Lysophospholipids/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Oxadiazoles/pharmacology
MH  - Propylene Glycols/pharmacology
MH  - Receptors, Lysosphingolipid/agonists/deficiency/*immunology
MH  - Sphingosine/*analogs & derivatives/immunology/pharmacology
MH  - Sphingosine-1-Phosphate Receptors
MH  - Thiophenes/pharmacology
EDAT- 2007/03/07 09:00
MHDA- 2007/05/23 09:00
CRDT- 2007/03/07 09:00
PHST- 2007/03/07 09:00 [pubmed]
PHST- 2007/05/23 09:00 [medline]
PHST- 2007/03/07 09:00 [entrez]
AID - 178/6/3437 [pii]
AID - 10.4049/jimmunol.178.6.3437 [doi]
PST - ppublish
SO  - J Immunol. 2007 Mar 15;178(6):3437-46. doi: 10.4049/jimmunol.178.6.3437.