PMID- 17339449
OWN - NLM
STAT- MEDLINE
DCOM- 20070522
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 6
DP  - 2007 Mar 15
TI  - Increased expression of leukocyte Ig-like receptor-1 and activating role of UL18 
      in the response to cytomegalovirus infection.
PG  - 3536-43
AB  - NK and T cells are important for combating CMV infection. Some NK and T cells 
      express leukocyte Ig-like receptor-1 (LIR-1), an inhibitory receptor recognizing 
      MHC class I and the CMV-encoded homolog UL18. We previously demonstrated an early 
      increase in LIR-1-expressing blood lymphocytes in lung-transplanted patients 
      later developing CMV disease. We now show that NK and T cells account for the 
      observed LIR-1 augmentation. Coincubation of PBMC from CMV-seropositive donors 
      with virus-infected lung fibroblasts led to a T cell-dependent secretion of 
      IFN-gamma, produced mainly by LIR-1(+) T cells and by NK cells. Cytokine 
      production during coculture with fibroblasts infected with virus containing the 
      UL18 gene was augmented compared with the UL18 deletion virus, suggesting a 
      stimulatory role for UL18. However, purified UL18Fc proteins inhibited IFN-gamma 
      production of LIR-1(+) T cells. We propose that cytokine production in the 
      transplant induces NK and T cells to express LIR-1, which may predispose to CMV 
      disease by MHC/LIR-1-mediated suppression. Although the UL18/LIR-1 interaction 
      could inhibit T cell responses, this unlikely plays a role in response to 
      infected cells. Instead, our data point to an activating role for viral UL18 
      during infection, where indirect intracellular effects cannot be excluded.
FAU - Wagner, Claudia S
AU  - Wagner CS
AD  - Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, 
      Karolinska University Hospital Huddinge, S-141 86 Stockholm, Sweden. 
      claudia.wagner@ki.se
FAU - Riise, Gerdt C
AU  - Riise GC
FAU - Bergstrom, Tomas
AU  - Bergstrom T
FAU - Karre, Klas
AU  - Karre K
FAU - Carbone, Ennio
AU  - Carbone E
FAU - Berg, Louise
AU  - Berg L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Capsid Proteins)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (VP23 protein, Human herpesvirus 1)
RN  - 0 (leukocyte-associated immunoglobulin-like receptor 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bystander Effect/immunology
MH  - Capsid Proteins/biosynthesis/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytomegalovirus/immunology
MH  - Cytomegalovirus Infections/etiology/*immunology/metabolism
MH  - Female
MH  - Fibroblasts/immunology/metabolism/virology
MH  - Histocompatibility Antigens Class I/immunology/metabolism
MH  - Humans
MH  - *Immune Tolerance
MH  - Immunity, Cellular
MH  - Killer Cells, Natural/immunology/metabolism
MH  - Lung Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Receptors, Immunologic/biosynthesis/*immunology
MH  - T-Lymphocytes/*immunology/metabolism
EDAT- 2007/03/07 09:00
MHDA- 2007/05/23 09:00
CRDT- 2007/03/07 09:00
PHST- 2007/03/07 09:00 [pubmed]
PHST- 2007/05/23 09:00 [medline]
PHST- 2007/03/07 09:00 [entrez]
AID - 178/6/3536 [pii]
AID - 10.4049/jimmunol.178.6.3536 [doi]
PST - ppublish
SO  - J Immunol. 2007 Mar 15;178(6):3536-43. doi: 10.4049/jimmunol.178.6.3536.