PMID- 17339488
OWN - NLM
STAT- MEDLINE
DCOM- 20070522
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 6
DP  - 2007 Mar 15
TI  - Variable requirement of dendritic cells for recruitment of NK and T cells to 
      different TLR agonists.
PG  - 3886-92
AB  - TLRs initiate the host immune response to microbial pathogens by activating cells 
      of the innate immune system. Dendritic cells (DCs) can be categorized into two 
      major groups, conventional DCs (including CD8(+) and CD8(-) DCs) and plasmacytoid 
      DCs. In mice, these subsets of DCs express a variety of TLRs, with conventional 
      DCs responding in vitro to predominantly TLR3, TLR4, TLR5, and TLR9 ligands, and 
      plasmacytoid DCs responding mainly to TLR7 and TLR9 ligands. However, the in vivo 
      requirement of DCs to initiate immune responses to specific TLR agonists is not 
      fully known. Using mice depleted of >90% of CD11c(+) MHC class II(+) DCs, we 
      demonstrate that cellular recruitment, including CD4(+) T cell and CX5(+)DX5(+) 
      NK cell recruitment to draining lymph nodes following the footpad administration 
      of TLR4 and TLR5 agonists, is dramatically decreased upon reduction of DC 
      numbers, but type I IFN production can partially substitute for DCs in response 
      to TLR3 and TLR7 agonists. Interestingly, TLR ligands can activate T cells and NK 
      cells in the draining lymph nodes, even with reduced DC numbers. The findings 
      reveal considerable plasticity in the response to TLR agonists, with TLR4 and 
      TLR5 agonists sharing the requirement of DCs for subsequent lymph node 
      recruitment of NK and T cells.
FAU - Uchida, Takefumi
AU  - Uchida T
AD  - Department of Surgery, University of Florida College of Medicine, 1600 SW Archer 
      Road, Gainesville, FL 32610, USA.
FAU - Scumpia, Philip O
AU  - Scumpia PO
FAU - Murasko, Donna M
AU  - Murasko DM
FAU - Seki, Shuhji
AU  - Seki S
FAU - Woulfe, Susan
AU  - Woulfe S
FAU - Clare-Salzler, Michael J
AU  - Clare-Salzler MJ
FAU - Moldawer, Lyle L
AU  - Moldawer LL
LA  - eng
GR  - R37 GM 40586/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CD11c Antigen)
RN  - 0 (Ligands)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology
MH  - CD8-Positive T-Lymphocytes/cytology/immunology
MH  - Cell Movement/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Killer Cells, Natural/cytology/*immunology
MH  - Ligands
MH  - Lymph Nodes/cytology/immunology
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plasma Cells/cytology/immunology
MH  - Toll-Like Receptors/*antagonists & inhibitors/*immunology
EDAT- 2007/03/07 09:00
MHDA- 2007/05/23 09:00
CRDT- 2007/03/07 09:00
PHST- 2007/03/07 09:00 [pubmed]
PHST- 2007/05/23 09:00 [medline]
PHST- 2007/03/07 09:00 [entrez]
AID - 178/6/3886 [pii]
AID - 10.4049/jimmunol.178.6.3886 [doi]
PST - ppublish
SO  - J Immunol. 2007 Mar 15;178(6):3886-92. doi: 10.4049/jimmunol.178.6.3886.