PMID- 17341037
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20131121
IS  - 0031-7144 (Print)
IS  - 0031-7144 (Linking)
VI  - 62
IP  - 2
DP  - 2007 Feb
TI  - Baicalein, a flavonoid extracted from a methanolic extract of Oroxylum indicum 
      inhibits proliferation of a cancer cell line in vitro via induction of apoptosis.
PG  - 149-53
AB  - A methanolic extract of the fruits of Oroxylum indicum, which is widely used in 
      traditional Chinese herbal medicine for its anti-inflammatory, anti-pyretic and 
      anti-hypersensitivity effects, inhibited in vitro proliferation of HL-60 cells. 
      The flavonoid baicalein was found as an active component in the extract. Analysis 
      of freeze-dried fruits of the plant indicated that this component comprised about 
      4% of the material by dry weight. In this study, we investigated the in vitro 
      effects of baicalein on the viability and induction of apoptosis in the HL-60 
      cell line. The cell viability after treating with baicalein for 24 h was 
      quantified by counting viable cells using trypan blue staining. The results 
      showed that baicalein caused a 50% inhibition of HL-60 cells at concentrations of 
      25-30 microM. The inhibition of proliferation of HL-60 cells due to 36-48 h 
      exposure to 10 or 20 microM baicalein was associated with the accumulation of 
      cells at S or G2M phases. However, proliferation inhibition at a higher dose may 
      be associated with induction by apoptosis, as evidenced by the typical nuclear 
      fragmentation using DNA fragmentation assay and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL). The results indicate that 
      baicalein has anti-tumor effects on human cancer cells, and Oroxylum indicum 
      extract could be used in supplementary cancer therapy.
FAU - Roy, M Kumar
AU  - Roy MK
AD  - National Food Research InstituteFood Function Laboratory, Tsukuba, Japan. 
      mkroy@affrc.go.jp
FAU - Nakahara, K
AU  - Nakahara K
FAU - Na, Thalang V
AU  - Na TV
FAU - Trakoontivakorn, G
AU  - Trakoontivakorn G
FAU - Takenaka, M
AU  - Takenaka M
FAU - Isobe, S
AU  - Isobe S
FAU - Tsushida, T
AU  - Tsushida T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pharmazie
JT  - Die Pharmazie
JID - 9800766
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Flavanones)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 49QAH60606 (baicalein)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Survival
MH  - Chromatography, High Pressure Liquid
MH  - DNA Fragmentation/drug effects
MH  - Flavanones/*pharmacology
MH  - Flow Cytometry
MH  - HL-60 Cells
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Methanol
MH  - Plant Extracts/chemistry/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - Solvents
MH  - Spectrophotometry, Ultraviolet
EDAT- 2007/03/08 09:00
MHDA- 2007/04/04 09:00
CRDT- 2007/03/08 09:00
PHST- 2007/03/08 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2007/03/08 09:00 [entrez]
PST - ppublish
SO  - Pharmazie. 2007 Feb;62(2):149-53.