PMID- 17343201
OWN - NLM
STAT- MEDLINE
DCOM- 20070831
LR  - 20190816
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 27
IP  - 10
DP  - 2006 Oct
TI  - [Combination of interphase- and metaphase-fluorescence in situ hybridization to 
      identify 11q23/MLL abnormalities in acute leukemia patients].
PG  - 682-6
AB  - OBJECTIVE: To explore a rapid, sensitive and effective method for identifying 11 
      q23/MLL gene rearrangements and investigate the incidence and clinical features 
      of adult acute leukemia (AL) patients with 11 q23/MLL abnormalities. METHODS: 
      Bone marrow samples from 112 adult AL patients were prepared by short-term (24 
      hours) unstimulated culture, and karyotyped by R-banding. The abnormal signals 
      were screened by interphase- fluorescence in situ hybridization (FISH) with 
      dual-color break-apart 11 q23/MLL-specific probe, and the 11 q23/MLL gene 
      rearrangements were determined by metaphase-FISH. RESULTS: Of the 112 patients,9 
      (8. 0%) with 11q23/MLL translocations were revealed by FISH, among which only 4 
      (3. 6% ) was revealed by CCA. Three patients were reported by CCA to have del( 
      11) ( q23) , while by FISH assay two of them were 11 q23/MLL translocation and 
      one was true deletion of I lq23 telomeric terminus. Furthermore by FISH assay II 
      q23/MLL translocations were identified in one each patient with normal karyotype, 
      with 11 q + and without overt 11 q23 abnormality. Eight patients with MLL gene 
      amplification including polysome, homogenous staining region (hsr) and double 
      minute chromosome (dmin) were also disclosed by FISH. AL patients with 11 q23/MLL 
      abnormalities were frequently diagnosed as pro-B acute lymphoblastic leukemia 
      (pro-B ALL) ,acute monocytic leukemia (AMoL) or biphenotypic acute leukemia 
      (BAL). CONCLUSION: FISH with dual-color break-apart I q23/MLL -specific probe is 
      a rapid and sensitive method to detect 11 q23/MLL abnormalities, as compared with 
      CCA. FISH also effectively discloses translocations and amplifications involving 
      11 q23/MLL,and should be performed in patients diagnosed as pro-B ALL,AMoL or 
      BAL, with CCA normal karyotype.
FAU - Zhao, Xi-chen
AU  - Zhao XC
AD  - Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, 
      China.
FAU - Li, Cheng-wen
AU  - Li CW
FAU - Dai, Yun
AU  - Dai Y
FAU - Liu, Xu-ping
AU  - Liu XP
FAU - Qin, Shuang
AU  - Qin S
FAU - Liu, Shi-he
AU  - Liu SH
FAU - Mi, Ying-chang
AU  - Mi YC
FAU - Wang, Jian-xiang
AU  - Wang JX
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (KMT2A protein, human)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Female
MH  - Gene Rearrangement
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Leukemia/*genetics
MH  - Male
MH  - Metaphase/genetics
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein/*genetics
MH  - Translocation, Genetic
EDAT- 2007/03/09 09:00
MHDA- 2007/09/01 09:00
CRDT- 2007/03/09 09:00
PHST- 2007/03/09 09:00 [pubmed]
PHST- 2007/09/01 09:00 [medline]
PHST- 2007/03/09 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2006 Oct;27(10):682-6.