PMID- 17345063
OWN - NLM
STAT- MEDLINE
DCOM- 20071212
LR  - 20220318
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 81
IP  - 9
DP  - 2007 Sep
TI  - Protective effects of ibuprofen on testicular torsion/detorsion-induced 
      ischemia/reperfusion injury in rats.
PG  - 655-63
AB  - The aim of this study was to investigate the protective effect of ibuprofen on 
      testicular torsion/detorsion-induced ischemia/reperfusion (I/R) injury. A total 
      of 48 prepubertal male Wistar albino rats were divided into two models: early and 
      late orchiectomy. Testicular torsion was created by rotating the right testis 720 
      degrees in a clockwise direction. The ischemia period was 5 h and orchiectomy was 
      performed after 5 h of detorsion in the early orchiectomy model (EOM). In the 
      late orchiectomy model (LOM), the ischemia period was 5 h and orchiectomy was 
      performed after 7 days of detorsion. In the EOM, ibuprofen (70 mg/kg, po) was 
      administrated only once, 40 min prior to detorsion. In the LOM, ibuprofen (70 
      mg/kg, po) was administered 40 min before detorsion, once daily for 7 days. 
      Bilateral orchiectomy was performed in all groups to measure the tissue levels of 
      malondialdehyde (MDA) and to microscopically investigate light and electrons. The 
      presence of endothelial nitric oxide synthase (eNOS) activity was shown with 
      immunohistochemical studies. Spermatogenesis and mean seminiferous tubule 
      diameter (MSTD) were significantly decreased in ipsilateral and contralateral 
      testis when both early and late I/R groups were compared to the sham groups. 
      Furthermore, ibuprofen-treated animals showed an improved histological appearance 
      in both models of testicular torsion. Ibuprofen treatment prevented lipid 
      peroxidation resulting in decreased MDA accumulation in the testes of both 
      models. After I/R, eNOS immunoreactivity was increased in the testicular tissues. 
      Ibuprofen treatment decreased eNOS immunoreactivity in the germ cells of the 
      tubules in the contralateral testes, but intense eNOS immunoreactivity was shown 
      in the ipsilateral testes of the LOM. Electron microscopy of the testes of rats 
      demonstrated that ibuprofen pretreatment was particularly effective in preventing 
      the mitochondrial degeneration in both Sertoli and spermatid cells in the LOM. 
      Because of its anti-inflammatory and antioxidant effects, ibuprofen pretreatment 
      may have protective effects in the experimental testicular torsion/detorsion 
      model in rats.
FAU - Dokmeci, Dikmen
AU  - Dokmeci D
AD  - Department of Pharmacology, Faculty of Medicine, Trakya University, Edirne, 
      Turkey.
FAU - Kanter, Mehmet
AU  - Kanter M
FAU - Inan, Mustafa
AU  - Inan M
FAU - Aydogdu, Nurettin
AU  - Aydogdu N
FAU - Basaran, Umit Nusret
AU  - Basaran UN
FAU - Yalcin, Omer
AU  - Yalcin O
FAU - Turan, Fatma Nesrin
AU  - Turan FN
LA  - eng
PT  - Journal Article
DEP - 20070308
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - WK2XYI10QM (Ibuprofen)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*therapeutic use
MH  - Antioxidants/*therapeutic use
MH  - Ibuprofen/*therapeutic use
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Nitric Oxide Synthase Type III
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*drug therapy/metabolism/pathology
MH  - Spermatic Cord Torsion/*drug therapy/metabolism/pathology
MH  - Testis/drug effects/metabolism/pathology/ultrastructure
EDAT- 2007/03/09 09:00
MHDA- 2007/12/13 09:00
CRDT- 2007/03/09 09:00
PHST- 2006/08/23 00:00 [received]
PHST- 2007/02/12 00:00 [accepted]
PHST- 2007/03/09 09:00 [pubmed]
PHST- 2007/12/13 09:00 [medline]
PHST- 2007/03/09 09:00 [entrez]
AID - 10.1007/s00204-007-0189-2 [doi]
PST - ppublish
SO  - Arch Toxicol. 2007 Sep;81(9):655-63. doi: 10.1007/s00204-007-0189-2. Epub 2007 
      Mar 8.