PMID- 17345115
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20181113
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 59
IP  - 4
DP  - 2007 Apr
TI  - Two variable lymphocyte receptor genes of the inshore hagfish are located far 
      apart on the same chromosome.
PG  - 329-31
AB  - Variable lymphocyte receptors (VLR) generate enormous diversity through 
      assembling highly diverse leucine-rich repeat (LRR) modules and presumably 
      function as antigen receptors in jawless vertebrates. The hagfish, which 
      constitute major extant members of jawless vertebrates along with lampreys, have 
      two VLR genes designated VLRA and VLRB, whereas only a single VLR gene has been 
      identified in the lamprey. In the present study, we show by fluorescence in situ 
      hybridization (FISH) that hagfish VLRA and VLRB are located on the same 
      chromosome, but are far apart from each other. Analysis of available inshore 
      hagfish complementary DNA sequences indicates that VLRA and VLRB do not share a 
      LRR module with an identical nucleotide sequence. Physical separation of VLRA and 
      VLRB is consistent with this observation and indicates that the two VLR genes 
      function as separate units. The FISH protocol developed in this study should be 
      useful for the analysis of the agnathan genome.
FAU - Kasamatsu, Jun
AU  - Kasamatsu J
AD  - Department of Pathology, Hokkaido University Graduate School of Medicine, 
      Sapporo, Japan.
FAU - Suzuki, Takashi
AU  - Suzuki T
FAU - Ishijima, Junko
AU  - Ishijima J
FAU - Matsuda, Yoichi
AU  - Matsuda Y
FAU - Kasahara, Masanori
AU  - Kasahara M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070214
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Receptors, Antigen)
SB  - IM
MH  - Animals
MH  - Chromosomes/genetics
MH  - Hagfishes/*genetics/immunology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/immunology
MH  - Petromyzon/genetics/immunology
MH  - Physical Chromosome Mapping
MH  - Receptors, Antigen/*genetics
EDAT- 2007/03/09 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/03/09 09:00
PHST- 2006/12/21 00:00 [received]
PHST- 2007/01/25 00:00 [accepted]
PHST- 2007/03/09 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/03/09 09:00 [entrez]
AID - 10.1007/s00251-007-0200-3 [doi]
PST - ppublish
SO  - Immunogenetics. 2007 Apr;59(4):329-31. doi: 10.1007/s00251-007-0200-3. Epub 2007 
      Feb 14.